Multi-ubiquitination of a nascent membrane protein produced in a rabbit reticulocyte lysate.

Multi-ubiquitination of a nascent membrane protein produced in a rabbit reticulocyte lysate. Research Abstract Details 

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Multi-ubiquitination of a nascent membrane protein produced in a rabbit reticulocyte lysate. Abstract Text:

S Iwamuro,M Saeki,S Kato,

During a large-scale in vitro translation analysis of a human full-length cDNA bank, we found many clones producing in vitro translation products showing ladder bands on a fluorogram with the equidistance of about 9 kDa at the position larger than the molecular mass expected from the open reading frame. We have analyzed a clone showing a typical pattern of the ladder bands. This clone encoded a 188-amino acid polypeptide containing a putative transmembrane domain. A green fluorescent protein-tagged polypeptide expressed in COS7 cells was localized in the endoplasmic reticulum and the Golgi apparatus. The ladder bands were observed in a rabbit reticulocyte lysate system, but not in a wheat germ extract system. Addition of the glutathione S-transferase-fused ubiquitin into the lysate caused upward shifts of the ladder bands. Addition of microsomal membranes prevented the formation of the ladder bands. Time course experiments demonstrated that the in vitro translation products increased in the presence of microsomal membranes, but were gradually degraded in their absence. These results suggest that the ladder formation resulted from the ubiquitination of misfolded polypeptide that failed to translocate to its proper position, and that an exclusion mechanism of misfolded membrane protein works in the rabbit reticulocyte lysate system.

Multi-ubiquitination of a nascent membrane protein produced in a rabbit reticulocyte lysate. Publishing Authors By Initials

S Iwamuro,M Saeki,S Kato,

For similar proteins: ubiquitins research abstracts see: proteins: ubiquitins research

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Multi-ubiquitination of a nascent membrane protein produced in a rabbit reticulocyte lysate. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 126

Page Numbers: 48-53

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jul

YEAR: 1999

Multi-ubiquitination of a nascent membrane protein produced in a rabbit reticulocyte lysate. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Multi-ubiquitination of a nascent membrane protein produced in a rabbit reticulocyte lysate. Keywords Mesh Terms:

KEYWORDS: Ubiquitins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Multi-ubiquitination of a nascent membrane protein produced in a rabbit reticulocyte lysate. Information

Substance Name: Proteasome Endopeptidase Complex

Registry Number: EC 3.4.25.1

Grant and Affiliation Information for Multi-ubiquitination of a nascent membrane protein produced in a rabbit reticulocyte lysate.

AFFILIATION: Kato Cytoprotein Network Project, ERATO, JST, c/o Sagami Chemical Research Center, Sagamihara, Kanagawa, 229-0012, Japan.

Country: JAPAN

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MEDLINETA: J Biochem

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ACCESSION NUMBER: AB020980

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