Multi-priming sequencing: a DNA sequencing method involving restriction enzyme-digested DNA fragments as primers.

Multi-priming sequencing: a DNA sequencing method involving restriction enzyme-digested DNA fragments as primers. Research Abstract Details 

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Multi-priming sequencing: a DNA sequencing method involving restriction enzyme-digested DNA fragments as primers. Abstract Text:

H Akiyama,S Kanai,H Ozawa,

An improved strategy for fluorescence-labeled dideoxy chain termination sequencing involving restriction enzyme-digested DNA fragments as primers, which are prepared from the DNA to be sequenced, is described. By using modified nucleoside triphosphates for strand protection in chain termination reactions, newly synthesized chains were detached from a primer at the regenerated recognition site by means of suitable restriction enzyme digestion. The digests could be analyzed with commercial automated DNA sequencers. Thus, by using restriction DNA fragments (double-stranded) as primers, sequence information was obtained from both "minus" and "plus" single-stranded DNA templates without subcloning. Nor is the synthesis of oligonucleotide primers needed. This method, named "Multi-Priming Sequencing," was proven to be time-saving, economical, and effective compared to conventional methods.

Multi-priming sequencing: a DNA sequencing method involving restriction enzyme-digested DNA fragments as primers. Publishing Authors By Initials

H Akiyama,S Kanai,H Ozawa,

For similar genetic structures: plasmids research abstracts see: genetic structures: plasmids research

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Multi-priming sequencing: a DNA sequencing method involving restriction enzyme-digested DNA fragments as primers. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 111

Page Numbers: 589-93

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 1992

Multi-priming sequencing: a DNA sequencing method involving restriction enzyme-digested DNA fragments as primers. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Multi-priming sequencing: a DNA sequencing method involving restriction enzyme-digested DNA fragments as primers. Keywords Mesh Terms:

KEYWORDS: Plasmids

MESH TERMS: genetics

Chemical & Substance for Abstract: Multi-priming sequencing: a DNA sequencing method involving restriction enzyme-digested DNA fragments as primers. Information

Substance Name: DNA Restriction Enzymes

Registry Number: EC 3.1.21.-

Grant and Affiliation Information for Multi-priming sequencing: a DNA sequencing method involving restriction enzyme-digested DNA fragments as primers.

AFFILIATION: Toray Research Center, Inc., Kanagawa.

Country: JAPAN

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MEDLINETA: J Biochem

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