Multi-channel counter-current chromatography for high-throughput fractionation of natural products for drug discovery.

Multi-channel counter-current chromatography for high-throughput fractionation of natural products for drug discovery. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Multi-channel counter-current chromatography for high-throughput fractionation of natural products for drug discovery. Abstract Text:

Shihua Wu,Lu Yang,Yuan Gao,Xiaoyue Liu,Feiyan Liu,

A multi-channel counter-current chromatography (CCC) method has been designed and fabricated for the high-throughput fractionation of natural products without complications sometimes encountered with other conventional chromatographic systems, such as irreversible adsorptive constituent losses and deactivation, tailing of solute peaks and contamination. It has multiple independent CCC channels and each channel connects independent separation column(s) by parallel flow tubes, and thus the multi-channel CCC apparatus can achieve simultaneously two or more independent chromatographic processes. Furthermore, a high-throughput CCC fractionation method for natural products has been developed by a combination of a new three-channel CCC apparatus and conventional parallel chromatographic devices including pumps, sample injectors, effluent detectors and collectors, and its performance has been displayed on the fractionation of ethyl acetate extracts of three natural materials Solidago canadensis, Suillus placidus, and Trichosanthes kirilowii, which are found to be potent cytotoxic to tumor cell lines in the course of screening the antitumor candidates. By combination of biological screening programs and preparative high-performance liquid chromatography (HPLC) purification, 22.8mg 6beta-angeloyloxykolavenic acid and 29.4mg 6beta-tigloyloxykolavenic acid for S. canadensis, 25.3mg suillin for S. placidus, and 6.8mg 23,24-dihydrocucurbitacin B for T. Kirilowii as their major cytotoxic principles were isolated from each 1000mg crude ethyl acetate exctract. Their chemical structures were characterized by electrospray ionization mass spectrometry, one- and two-dimensional nuclear magnetic resonance. The overall results indicate the multi-channel CCC is very useful for high-throughput fractionation of natural products for drug discovery in spite of the solvent balancing requirement and the lower resolution of the shorter CCC columns.

Multi-channel counter-current chromatography for high-throughput fractionation of natural products for drug discovery. Publishing Authors By Initials

S Wu,L Yang,Y Gao,X Liu,F Liu,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Multi-channel counter-current chromatography for high-throughput fractionation of natural products for drug discovery. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of chromatography. A

VOLUME: 1180

Page Numbers: 99-107

Journal Abbreviation:

ISSN: 0021-9673

DAY: 31

MONTH: 12

YEAR: 2007

Multi-channel counter-current chromatography for high-throughput fractionation of natural products for drug discovery. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9318488

Multi-channel counter-current chromatography for high-throughput fractionation of natural products for drug discovery. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Multi-channel counter-current chromatography for high-throughput fractionation of natural products for drug discovery. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Multi-channel counter-current chromatography for high-throughput fractionation of natural products for drug discovery.

AFFILIATION: Zhejiang University, Research Center of Siyuan Natural Pharmacy and Biotoxicology, College of Life Sciences, Zijinggang Campus, Hangzhou 310058, China.

Country: Netherlands

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Chromatogr A

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Multi-channel counter-current chromatography for high-throughput fractionation of natural products for drug discovery Related Publications

• Altered mucin gene expression in stone-containing intrahepatic bile ducts and cholangiocarcinomas.
• Clinical study on labor pain relief using the combined spinal-epidural analgesia and inhaling nitrous oxide.
• Detection of cariogenic Streptococcus mutans by quantitative polymerase chain reaction.
• Diagnosis and treatment of primary gastric non-Hodgkin's lymphoma: analysis of 157 patients.
• Experimental study on the effect of cyclosporine A drug delivery system implanted in anterior chamber on high-risk corneal graft rejection.
• Inhibitory effects of RRR-alpha-tocopheryl succinate on benzo(a)pyrene (B(a)P)-induced forestomach carcinogenesis in female mice.
• Mechanism and treatments of regression and haze after photorefractive keratectomy.
• Reconstruction and transplantation of composite skin containing keratinocytes and fibroblasts.
• Studies of WT1 gene expression in leukemia patients.
• [A case report of primary sclerosing cholangiolitis with Budd-Chiari syndrome]
• [A study on the epidemiological characteristics and the preventive measures of rabies in Shanghai]
• [Clinical and pathological characteristics of primary biliary cirrhosis]
• [Clinical results of transmyocardial laser revascularization for 77 patients with coronary artery disease]
• [Difference in action sites between mecamylamine and hexamethonium on nicotinic receptors of sympathetic neurons]
• [Differences of nitric oxide synthase expression and activity between splanchnic arterial and venous vessels of cirrhotic rats]
• [Effects of recombinant human bone morphogenic protein-2 and hyaluronic acid on invasion of brain glioma in vivo]
• [Improved three-dimension reconstruction of serial frozen tissue sections of the temporal bone of guinea pigs]
• [Measurement of anti-acetaldehyde protein adduct IgA, IgG, IgM antibody titers in the sera of human alcoholics]
• [Recent research advances in oxidized high density lipoproteins]
• [Regulation of c-myc expression in leukemic cell line Meg-01 by the death domain of Fas gene]
• [Relationship between the proliferative activity of cancer cells and microvessel density in portal vein thrombosis and transfer of hepatocellular carcinoma]
• [Studies on cell transformation and cell cycle in human embryo lungs induced by nickel compounds]
• [Study on the epidemiological feature and control measure for rabies from 1990 to 2000 in Jiangsu province]
• [Surgical treatment of epispadias]
• [The application of colour Doppler and ultrasonography the grading and staging of chronic hepatitis with and without liver cirrhosis]
• [The determination of anions of very weak acids by incompletely suppressed conductometric detection]
• [The experimental study on the activity of rhBMP-2, coral and collagen composites inducing intramuscle bone]
• [The expression of P16, P21(WAF1/CIP1) and Cyclin D1 proteins in skin of patients with arseniasis caused by coal-burning and their significance]
• [The sinus node itself also plays a role in heart rate slowing down during postnatal development]
• [The ultrastructure of bone morphogenetic protein 2 gene transfected human periodontal ligament fibroblasts]