Mucin coatings suppress neutrophil adhesion to a polymeric model biomaterial.

Mucin coatings suppress neutrophil adhesion to a polymeric model biomaterial. Research Abstract Details 

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Mucin coatings suppress neutrophil adhesion to a polymeric model biomaterial. Abstract Text:

Tomas Sandberg,Jan Carlsson,Marjam Karlsson Ott,Tomas Sandberg,Jan Carlsson,Marjam Karlsson Ott,

Following our recent study on the fractionation, characterization, and model adsorption of mucins derived from bovine salivary glands (BSM), porcine stomach scrapings (PGM), and human whole saliva (MG1), we here present a microscopic evaluation of the interactions between mucin-coated substrates and human neutrophils. Our results show that surface-coating with BSM, PGM, and MG1 can be effectively used to suppress the adhesion of neutrophils to a polymeric model biomaterial (Thermanox). Neutrophil morphologies found on Thermanox substrates coated with mucins resemble those observed for nonactivated neutrophils found in circulation. Notably, low neutrophil adhesion can be obtained at a significantly lower coating concentration (0.125 mg/mL) for the compositionally complex MG1 mucin than for the relatively pure BSM and PGM mucins (1 mg/mL). Furthermore, since coating at a low BSM and PGM concentration (0.25 mg/mL) results in higher cell counts and more spread cells than in the high-concentration case, we suggest that dense mucin surface packing is critical for good coating performance. In conclusion, the present study demonstrates how mucins from three different sources, of different compositional and structural status, efficiently can be used to suppress neutrophil adhesion and activation. This finding makes them potent candidates for use as biomaterial coatings.

Mucin coatings suppress neutrophil adhesion to a polymeric model biomaterial. Publishing Authors By Initials

T Sandberg,J Carlsson,MK Ott,T Sandberg,J Carlsson,MK Ott,

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Mucin coatings suppress neutrophil adhesion to a polymeric model biomaterial. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Microscopy research and technique

VOLUME: 70

Page Numbers: 864-8

Journal Abbreviation: Microsc. Res. Tech.

ISSN: 1059-910X

DAY: 25

MONTH: Oct

YEAR: 2007

Mucin coatings suppress neutrophil adhesion to a polymeric model biomaterial. Information

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LANGUAGE: eng

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AFFILIATION: Division of Surface Biotechnology, Department of Physical and Analytical Chemistry, BMC, Uppsala University, SE-751 23 Uppsala, Sweden. tomas.sandberg@ytbioteknik.uu.se

Country: United States

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MEDLINETA: Microsc Res Tech

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