MUC1-derived glycopeptide libraries with improved MHC anchors are strong antigens and prime mouse T cells for proliferative responses to lysates of human breast cancer tissue.

MUC1-derived glycopeptide libraries with improved MHC anchors are strong antigens and prime mouse T cells for proliferative responses to lysates of human breast cancer tissue. Research Abstract Details 

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MUC1-derived glycopeptide libraries with improved MHC anchors are strong antigens and prime mouse T cells for proliferative responses to lysates of human breast cancer tissue. Abstract Text:

Monika Gad,Teis Jensen,Rod Gagne,Shiro Komba, Daugaard,Niels Kroman,Morten Meldal,Ole Werdelin,

Multi-component glycopeptide libraries and single glycopeptides were used for immunization of mice with the aim of inducing strong T helper cell responses to the repetitive sequence of MUC1 expressed by human tumor cells. The glycopeptides and glycopeptide libraries were modeled upon the native human MUC1 amino acid variable number of tandem repeats sequence by introduction of modifications in the MHC anchor positions to optimally fulfil the binding requirements of the A(d) MHC class II molecule in the BALB/c mouse. The immunogenicity of the MUC1 glycopeptides in BALB/c mice was determined by immunization in complete Freund's adjuvant and assaying lymph node T cells for a proliferative response to the glycopeptide used. Strong proliferative responses with stimulation indices over 50 were obtained with anchor-improved glycopeptide libraries as well as with single glycopeptides. Immunization with one of the glycopeptide libraries primed T cells for a proliferative cross-response to the native MUC1 glycopeptide, which by itself was nonimmunogenic. In addition, immunization with the same glycopeptide library primed T cells for a strong response to lysate of a MUC1-expressing human breast cancer, and immunization with the tumor lysate primed T cells for a response to the glycopeptide library. The T cells responding in the assay for proliferation were restricted to the A(d) MHC class II molecule. The results indicate that immunization with MHC anchor-improved MUC1 glycopeptide libraries can effectively prime T helper cells and may induce long-term memory. The approach may be useful in the design of preventive cancer vaccines for use in humans.

MUC1-derived glycopeptide libraries with improved MHC anchors are strong antigens and prime mouse T cells for proliferative responses to lysates of human breast cancer tissue. Publishing Authors By Initials

M Gad,T Jensen,R Gagne,S Komba,S Daugaard,N Kroman,M Meldal,O Werdelin,

For similar complex mixtures: tissue extracts research abstracts see: complex mixtures: tissue extracts research

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MUC1-derived glycopeptide libraries with improved MHC anchors are strong antigens and prime mouse T cells for proliferative responses to lysates of human breast cancer tissue. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: European journal of immunology

VOLUME: 33

Page Numbers: 1624-32

Journal Abbreviation: Eur. J. Immunol.

ISSN: 0014-2980

DAY: 1

MONTH: Jun

YEAR: 2003

MUC1-derived glycopeptide libraries with improved MHC anchors are strong antigens and prime mouse T cells for proliferative responses to lysates of human breast cancer tissue. Information

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LANGUAGE: eng

NlmUniqueID: 1273201

MUC1-derived glycopeptide libraries with improved MHC anchors are strong antigens and prime mouse T cells for proliferative responses to lysates of human breast cancer tissue. Keywords Mesh Terms:

KEYWORDS: Tissue Extracts

MESH TERMS: immunology

Chemical & Substance for Abstract: MUC1-derived glycopeptide libraries with improved MHC anchors are strong antigens and prime mouse T cells for proliferative responses to lysates of human breast cancer tissue. Information

Substance Name: Tissue Extracts

Registry Number: 0

Grant and Affiliation Information for MUC1-derived glycopeptide libraries with improved MHC anchors are strong antigens and prime mouse T cells for proliferative responses to lysates of human breast cancer tissue.

AFFILIATION: Institute for Medical Microbiology and Immunology, University of Copenhagen, Denmark.

Country: Germany

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MEDLINETA: Eur J Immunol

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