MRI detection of macrophages labeled using micrometer-sized iron oxide particles.

MRI detection of macrophages labeled using micrometer-sized iron oxide particles. Research Abstract Details 

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MRI detection of macrophages labeled using micrometer-sized iron oxide particles. Abstract Text:

John B Williams,Qing Ye,T Kevin Hitchens,Christina L Kaufman,Chien Ho,

PURPOSE: To evaluate cellular labeling of immune cells using micron-sized iron oxide particles (MPIOs) and evaluate the MR relaxivity and MRI detection of the labeled cells. MATERIALS AND METHODS: Immune cells isolated from mice and rats were labeled with three different sizes of MPIO particles (0.35, 0.90, or 1.63 microm). These labeled cells were characterized using transmission electron microscopy (TEM), fluorescence microscopy, flow cytometry, MR relaxometry, and MRI. RESULTS: Macrophage uptake of MPIOs was found to be highest for the 1.63-microm size particles. MR relaxivity measurements indicated greater spin-spin relaxation for MPIO-labeled cells relative to cells labeled with nanometer-sized ultra-small superparamagnetic iron oxide (USPIO) particles with similar iron content. TEM and fluorescence microscopy indicated cellular uptake of multiple MPIO particles per cell. Macrophages labeled with 1.63-microm MPIOs had an average cellular iron uptake of 39.1 pg/cell, corresponding to approximately 35 particles per cell. CONCLUSION: Cells labeled with one or more MPIO particles could be readily detected ex vivo at 11.7 Tesla and after infusion of the MPIO-labeled macrophages into the kidney of a rat, hypointense regions of the outer cortex are observed, in vivo, by MRI at 4.7 Tesla.

MRI detection of macrophages labeled using micrometer-sized iron oxide particles. Publishing Authors By Initials

JB Williams,Q Ye,TK Hitchens,CL Kaufman,C Ho,

For similar investigative techniques: clinical laboratory techniques: cytological techniques: histocytological preparation techniques: staining and labeling research abstracts see: investigative techniques: clinical laboratory techniques: cytological techniques: histocytological preparation techniques: staining and labeling research

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MRI detection of macrophages labeled using micrometer-sized iron oxide particles. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of magnetic resonance imaging : JMRI

VOLUME: 25

Page Numbers: 1210-8

Journal Abbreviation:

ISSN: 1053-1807

DAY: 3

MONTH: Jun

YEAR: 2007

MRI detection of macrophages labeled using micrometer-sized iron oxide particles. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9105850

MRI detection of macrophages labeled using micrometer-sized iron oxide particles. Keywords Mesh Terms:

KEYWORDS: Staining and Labeling

MESH TERMS: pharmacokinetics

Chemical & Substance for Abstract: MRI detection of macrophages labeled using micrometer-sized iron oxide particles. Information

Substance Name: Iron

Registry Number: 7439-89-6

Grant and Affiliation Information for MRI detection of macrophages labeled using micrometer-sized iron oxide particles.

AFFILIATION: Pittsburgh NMR Center for Biomedical Research, Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA.

Country: United States

AGENCY: United States NCRR

GRANT: S10 RR 15704

ACRONYM: RR

MEDLINETA: J Magn Reson Imaging

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