Mouse R-spondin2 is required for apical ectodermal ridge maintenance in the hindlimb.

Mouse R-spondin2 is required for apical ectodermal ridge maintenance in the hindlimb. Research Abstract Details 

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Mouse R-spondin2 is required for apical ectodermal ridge maintenance in the hindlimb. Abstract Text:

Ju-Suk Nam,Emily Park,Taryn J Turcotte,Servando Palencia,Xiaoming Zhan,Jackie Lee,Kyuson Yun,Walter D Funk,Jeong Kyo Yoon,

The R-spondin (Rspo) family of proteins consists of secreted cysteine-rich proteins that can activate beta-catenin signaling via the Frizzled/LRP5/6 receptor complex. Here, we report that targeted inactivation of the mouse Rspo2 gene causes developmental limb defects, especially in the hindlimb. Although the initiation of the expression of apical ectodermal ridge (AER)-specific genes, including fibroblast growth factor 8 (FGF8) and FGF4 occurred normally, the maintenance of these marker expressions was significantly defective in the hindlimb of Rspo2(-/-) mice. Consistent with the ligand role of R-spondins in the Wnt/beta-catenin signaling pathway, expression of Axin2 and Sp8, targets for beta-catenin signaling, within AER was greatly reduced in Rspo2(-/-) embryos. Furthermore, sonic hedgehog (Shh) signaling within the hindlimbs of Rspo2(-/-) mice was also significantly decreased. Rspo2 is expressed in the AER of all limb buds, however the stunted phenotype is significantly more severe in the hindlimbs than the forelimbs and strongly biased to the left side. Our findings strongly suggest that Rspo2 expression in the AER is required for AER maintenance likely by regulating Wnt/beta-catenin signaling.

Mouse R-spondin2 is required for apical ectodermal ridge maintenance in the hindlimb. Publishing Authors By Initials

JS Nam,E Park,TJ Turcotte,S Palencia,X Zhan,J Lee,K Yun,WD Funk,JK Yoon,

For similar peptides: intercellular signaling peptides and proteins: wnt proteins research abstracts see: peptides: intercellular signaling peptides and proteins: wnt proteins research

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Mouse R-spondin2 is required for apical ectodermal ridge maintenance in the hindlimb. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Developmental biology

VOLUME: 311

Page Numbers: 124-35

Journal Abbreviation: Dev. Biol.

ISSN: 1095-564X

DAY: 17

MONTH: 08

YEAR: 2007

Mouse R-spondin2 is required for apical ectodermal ridge maintenance in the hindlimb. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 372762

Mouse R-spondin2 is required for apical ectodermal ridge maintenance in the hindlimb. Keywords Mesh Terms:

KEYWORDS: Wnt Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Mouse R-spondin2 is required for apical ectodermal ridge maintenance in the hindlimb. Information

Substance Name: Wnt Proteins

Registry Number: 0

Grant and Affiliation Information for Mouse R-spondin2 is required for apical ectodermal ridge maintenance in the hindlimb.

AFFILIATION: Center for Molecular Medicine, Maine Medical Center Research Institute, 81 Research Drive, Scarborough, ME 04074, USA.

Country: United States

AGENCY: United States NCRR

GRANT: P20 RR018789

ACRONYM: RR

MEDLINETA: Dev Biol

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