Mouse pachytene checkpoint 2 (trip13) is required for completing meiotic recombination but not synapsis.

Mouse pachytene checkpoint 2 (trip13) is required for completing meiotic recombination but not synapsis. Research Abstract Details 

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Mouse pachytene checkpoint 2 (trip13) is required for completing meiotic recombination but not synapsis. Abstract Text:

Xin Chenglin Li,Xin Li,John C Schimenti,Xin Chenglin Li,Xin Li,John C Schimenti,

In mammalian meiosis, homologous chromosome synapsis is coupled with recombination. As in most eukaryotes, mammalian meiocytes have checkpoints that monitor the fidelity of these processes. We report that the mouse ortholog (Trip13) of pachytene checkpoint 2 (PCH2), an essential component of the synapsis checkpoint in Saccharomyces cerevisiae and Caenorhabditis elegans, is required for completion of meiosis in both sexes. TRIP13-deficient mice exhibit spermatocyte death in pachynema and loss of oocytes around birth. The chromosomes of mutant spermatocytes synapse fully, yet retain several markers of recombination intermediates, including RAD51, BLM, and RPA. These chromosomes also exhibited the chiasmata markers MLH1 and MLH3, and okadaic acid treatment of mutant spermatocytes caused progression to metaphase I with bivalent chromosomes. Double mutant analysis demonstrated that the recombination and synapsis genes Spo11, Mei1, Rec8, and Dmc1 are all epistatic to Trip13, suggesting that TRIP13 does not have meiotic checkpoint function in mice. Our data indicate that TRIP13 is required after strand invasion for completing a subset of recombination events, but possibly not those destined to be crossovers. To our knowledge, this is the first model to separate recombination defects from asynapsis in mammalian meiosis, and provides the first evidence that unrepaired DNA damage alone can trigger the pachytene checkpoint response in mice.

Mouse pachytene checkpoint 2 (trip13) is required for completing meiotic recombination but not synapsis. Publishing Authors By Initials

XC Li,X Li,JC Schimenti,XC Li,X Li,JC Schimenti,

For similar urogenital system: genitalia: germ cells: spermatozoa: spermatocytes research abstracts see: urogenital system: genitalia: germ cells: spermatozoa: spermatocytes research

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Mouse pachytene checkpoint 2 (trip13) is required for completing meiotic recombination but not synapsis. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: PLoS genetics

VOLUME: 3

Page Numbers: e130

Journal Abbreviation: PLoS Genet.

ISSN: 1553-7404

DAY: 21

MONTH: 06

YEAR: 2007

Mouse pachytene checkpoint 2 (trip13) is required for completing meiotic recombination but not synapsis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101239074

Mouse pachytene checkpoint 2 (trip13) is required for completing meiotic recombination but not synapsis. Keywords Mesh Terms:

KEYWORDS: Spermatocytes

MESH TERMS: metabolism

Chemical & Substance for Abstract: Mouse pachytene checkpoint 2 (trip13) is required for completing meiotic recombination but not synapsis. Information

Substance Name: pachytene checkpoint 2 protein, mouse

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for Mouse pachytene checkpoint 2 (trip13) is required for completing meiotic recombination but not synapsis.

AFFILIATION: Cornell University, College of Veterinary Medicine, Department of Biomedical Sciences, Ithaca, New York, United States of America.

Country: United States

AGENCY: United States NIGMS

GRANT: GM45415

ACRONYM: GM

MEDLINETA: PLoS Genet

REFSOURCE: PLoS Genet. 2007 Sep 7;3(9):e168

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