Mouse embryonic stem cells form follicle-like ovarian structures but do not progress through meiosis.

Mouse embryonic stem cells form follicle-like ovarian structures but do not progress through meiosis. Research Abstract Details 

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Mouse embryonic stem cells form follicle-like ovarian structures but do not progress through meiosis. Abstract Text:

Ivana Novak,Daniel A Lightfoot,Hong Wang,Annika Eriksson,Ensaf Mahdy,Christer ,Ivana Novak,Daniel A Lightfoot,Hong Wang,Annika Eriksson,Ensaf Mahdy,Christer ,

Several recent studies have suggested that mouse embryonic stem cells (ESCs) can differentiate into female and male germ cells in vitro. The meiotic process in germ cell-like cells derived from ESCs has not been studied in detail, but it has been reported that synaptonemal complex protein-3 (SYCP3) is expressed in these cells. Here, we have carefully evaluated the meiotic process in germ cell-like cells derived from ESCs, using a panel of meiosis-specific markers that identify distinct meiotic signatures unique to meiotic prophase I development in vivo. We find that whereas SYCP3 is expressed in germ cell-like cells, other meiotic proteins, such as SYCP1, SYCP2, STAG3 (stromal antigen 3), REC8 (meiotic protein similar to the rad21 cohesins), and SMC1 (structural maintenance of chromosomes-1)-beta, are not expressed. The nuclear distribution of SYCP3 in the germ cell-like cells is highly abnormal and not associated with the chromosomes of these cells. Fluorescence in situ hybridization analysis shows that the SYCP3-positive germ cell-like cells do not contain synapsed homologous chromosomes but instead display a chromosomal organization normally found in somatic cells. The absence of expression of essential meiotic proteins and a normal meiotic chromosomal organization strongly suggests that the germ cell-like cells formed from ESCs fail to progress through meiosis.

Mouse embryonic stem cells form follicle-like ovarian structures but do not progress through meiosis. Publishing Authors By Initials

I Novak,DA Lightfoot,H Wang,A Eriksson,E Mahdy,C ,I Novak,DA Lightfoot,H Wang,A Eriksson,E Mahdy,C ,

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Mouse embryonic stem cells form follicle-like ovarian structures but do not progress through meiosis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Stem cells (Dayton, Ohio)

VOLUME: 24

Page Numbers: 1931-6

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ISSN: 1066-5099

DAY: 27

MONTH: 04

YEAR: 2006

Mouse embryonic stem cells form follicle-like ovarian structures but do not progress through meiosis. Information

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LANGUAGE: eng

NlmUniqueID: 9304532

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Grant and Affiliation Information for Mouse embryonic stem cells form follicle-like ovarian structures but do not progress through meiosis.

AFFILIATION: Department of Cell and Molecular Biology, Karolinska Institutet, SE-171 77, Stockholm, Sweden.

Country: United States

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MEDLINETA: Stem Cells

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