Motile human normozoospermic and oligozoospermic semen samples show a difference in double-strand DNA break incidence.

Motile human normozoospermic and oligozoospermic semen samples show a difference in double-strand DNA break incidence. Research Abstract Details 

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Motile human normozoospermic and oligozoospermic semen samples show a difference in double-strand DNA break incidence. Abstract Text:

Alwin A H A Derijck,Godfried W van der Heijden,Liliana Ramos,Maud Giele,Jan A M Kremer,Peter de Boer,

BACKGROUND: Among ICSI children de novo structural chromosome aberrations of male descent are increased. Misrepair of double-strand DNA breaks (DSBs) is a prerequisite for such aberrations to occur. To date, no absolute assessment of the number of DSBs in human sperm nuclei after gamete fusion has been described. METHODS: Using man-mouse heterologous ICSI and gammaH2AX immunofluorescent staining, capable of detecting a single DSB, the number of lesions in ICSI selected sperm from normozoospermic men (n = 2) and oligozoospermic patients (n = 3) was quantified. A comparison with a subfertile male mouse model (n = 5) has been made. In addition, the fate of morphologically normal ejaculated immotile sperm after ICSI was examined. RESULTS: A significant increase in the fraction of sperm cells bearing DSBs was found in oligozoospermic semen compared with that from normozoospermic men (P < 0.01). The majority of morphologically normal immotile human sperm showed excess gammaH2AX staining and nuclear disintegration. However, some had a non-deviant DSB pattern. CONCLUSIONS: The increased fraction of DSB-positive sperm in both human and mouse oligozoospermic semen is adding to the surmise that semen from oligozoospermic patients has a reduced chromatin quality, causally related to reduced preimplantation embryo development. The use of ejaculated immotile sperm for in vitro reproduction is debatable due to sperm DNA degradation.

Motile human normozoospermic and oligozoospermic semen samples show a difference in double-strand DNA break incidence. Publishing Authors By Initials

AA Derijck,GW van der Heijden,L Ramos,M Giele,JA Kremer,P de Boer,

For similar biological phenomena, cell phenomena, and immunity: cell physiology: cell movement: sperm motility research abstracts see: biological phenomena, cell phenomena, and immunity: cell physiology: cell movement: sperm motility research

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Motile human normozoospermic and oligozoospermic semen samples show a difference in double-strand DNA break incidence. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Human reproduction (Oxford, England)

VOLUME: 22

Page Numbers: 2368-76

Journal Abbreviation: Hum. Reprod.

ISSN: 0268-1161

DAY: 18

MONTH: 06

YEAR: 2007

Motile human normozoospermic and oligozoospermic semen samples show a difference in double-strand DNA break incidence. Information

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LANGUAGE: eng

NlmUniqueID: 8701199

Motile human normozoospermic and oligozoospermic semen samples show a difference in double-strand DNA break incidence. Keywords Mesh Terms:

KEYWORDS: Sperm Motility

MESH TERMS: genetics

Chemical & Substance for Abstract: Motile human normozoospermic and oligozoospermic semen samples show a difference in double-strand DNA break incidence. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Motile human normozoospermic and oligozoospermic semen samples show a difference in double-strand DNA break incidence.

AFFILIATION: Department of Obstetrics and Gynaecology, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands.

Country: England

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MEDLINETA: Hum Reprod

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