Monocytes, but not T or B cells, are the principal target cells for dengue virus (DV) infection among human peripheral blood mononuclear cells.

Monocytes, but not T or B cells, are the principal target cells for dengue virus (DV) infection among human peripheral blood mononuclear cells. Research Abstract Details 

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Monocytes, but not T or B cells, are the principal target cells for dengue virus (DV) infection among human peripheral blood mononuclear cells. Abstract Text:

Zhihua Kou,Matthew Quinn,Huiyuan Chen,W W Shanaka I Rodrigo,Robert C Rose,Jacob J Schlesinger,Xia Jin,Zhihua Kou,Matthew Quinn,Huiyuan Chen,W W Shanaka I Rodrigo,Robert C Rose,Jacob J Schlesinger,Xia Jin,

A better understanding of the pathogenesis of dengue hemorrhagic fever and dengue shock syndrome requires the precise identification of dengue virus (DV) permissive target cells. To examine the relative DV permissiveness among cell subsets, we inoculated unfractionated human peripheral blood mononuclear cells with DV2-16681 in the presence or absence of pooled DV-immune human sera (PHS), and assessed infection with fluorescent dye labeled DV-specific monoclonal antibody and cell surface markers using flow cytometry. We found significantly higher levels of DV antigen staining on DV-infected than mock-infected primary monocytes (3.54 +/- 3.42% vs. 0.50 +/- 0.38%; P = 0.001). The magnitude of infection was markedly enhanced in the presence of highly diluted PHS (10.04 +/- 6.10% vs. 3.54 +/- 3.42%; P = 0.015). Under identical experimental conditions, primary T or B cells were not infected either with or without the addition of PHS (0.06 +/- 0.04% and 0.44 +/- 0.22% for T and B cells, respectively). Furthermore, depletion of CD14+ monocytes prior to DV inoculation abrogated the detection of infected cells, and the addition of monoclonal antibodies to either FcgammaRI (CD64) or FcgammaRII (CD32) led to a 50-70% reduction in antibody-dependent enhancement (ADE) of DV infection. Collectively, these results provide further support to the notion that primary monocytes and FcgammaRs expressed on these cells may be important in the initial steps of immune enhancement observed in some patients with natural DV infection. They also demonstrate that using modern experimental technology, DV infection, and neutralization and enhancement of DV infection can be easily assessed simultaneously in multiple cell types.

Monocytes, but not T or B cells, are the principal target cells for dengue virus (DV) infection among human peripheral blood mononuclear cells. Publishing Authors By Initials

Z Kou,M Quinn,H Chen,WW Rodrigo,RC Rose,JJ Schlesinger,X Jin,Z Kou,M Quinn,H Chen,WW Rodrigo,RC Rose,JJ Schlesinger,X Jin,

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Monocytes, but not T or B cells, are the principal target cells for dengue virus (DV) infection among human peripheral blood mononuclear cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of medical virology

VOLUME: 80

Page Numbers: 134-46

Journal Abbreviation: J. Med. Virol.

ISSN: 0146-6615

DAY: 6

MONTH: Jan

YEAR: 2008

Monocytes, but not T or B cells, are the principal target cells for dengue virus (DV) infection among human peripheral blood mononuclear cells. Information

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LANGUAGE: eng

NlmUniqueID: 7705876

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Grant and Affiliation Information for Monocytes, but not T or B cells, are the principal target cells for dengue virus (DV) infection among human peripheral blood mononuclear cells.

AFFILIATION: Department of Medicine, Infectious Diseases Division, University of Rochester, Rochester, New York 14642, USA.

Country: United States

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MEDLINETA: J Med Virol

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