Monoclonal antibodies toward scallop (Patinopecten yessoensis) testis and wheat germ calmodulins.

Monoclonal antibodies toward scallop (Patinopecten yessoensis) testis and wheat germ calmodulins. Research Abstract Details 

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Monoclonal antibodies toward scallop (Patinopecten yessoensis) testis and wheat germ calmodulins. Abstract Text:

K Kobayashi,M Yoshida,Y Shinoda,M Yazawa,K Yagi,

A monoclonal antibody (IM7) toward scallop testis calmodulin and another one (PBE2) toward wheat germ calmodulin were produced. Ca2+ was required for IM7 to react with scallop calmodulin. IM7 reacted with the C-terminal region (Asp78-Lys148) of the calmodulin. As observed on competitive ELISA, IM7 reacted with chicken calmodulin, but not with Euglena gracilis or wheat calmodulin, troponin C, myosin light chains, or parvalbumin. It is assumed that the cluster of Thr143, Thr146, and Ser147 in the C-terminal region acts as the antigenic site. IM7 (and Fab of IM7) inhibited the activities of myosin light chain kinase and cAMP-phosphodiesterase. PBE2 reacted with wheat germ calmodulin irrespective of the presence or absence of Ca2+, the antigenic site being in the N-terminal region (Ala1-Met37). It reacted with wheat and spinach calmodulins, but not with scallop, chicken, or Euglena calmodulin, troponin C, myosin light chains, or parvalbumin. PBE2 had no effect on the activities of myosin light chain kinase and cAMP-phosphodiesterase.

Monoclonal antibodies toward scallop (Patinopecten yessoensis) testis and wheat germ calmodulins. Publishing Authors By Initials

K Kobayashi,M Yoshida,Y Shinoda,M Yazawa,K Yagi,

For similar plants: plant families and groups: angiosperms: poaceae: triticum research abstracts see: plants: plant families and groups: angiosperms: poaceae: triticum research

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Monoclonal antibodies toward scallop (Patinopecten yessoensis) testis and wheat germ calmodulins. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 109

Page Numbers: 551-8

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Apr

YEAR: 1991

Monoclonal antibodies toward scallop (Patinopecten yessoensis) testis and wheat germ calmodulins. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Monoclonal antibodies toward scallop (Patinopecten yessoensis) testis and wheat germ calmodulins. Keywords Mesh Terms:

KEYWORDS: Triticum

MESH TERMS: metabolism

Chemical & Substance for Abstract: Monoclonal antibodies toward scallop (Patinopecten yessoensis) testis and wheat germ calmodulins. Information

Substance Name: 3',5'-Cyclic-AMP Phosphodiesterases

Registry Number: EC 3.1.4.17

Grant and Affiliation Information for Monoclonal antibodies toward scallop (Patinopecten yessoensis) testis and wheat germ calmodulins.

AFFILIATION: Department of Chemistry, Faculty of Science, Hokkaido University.

Country: JAPAN

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MEDLINETA: J Biochem

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