Molecular profiling of angiogenesis with targeted ultrasound imaging: early assessment of antiangiogenic therapy effects.

Molecular profiling of angiogenesis with targeted ultrasound imaging: early assessment of antiangiogenic therapy effects. Research Abstract Details 

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Molecular profiling of angiogenesis with targeted ultrasound imaging: early assessment of antiangiogenic therapy effects. Abstract Text:

Moritz Palmowski,Jochen Huppert,Gesa Ladewig,Peter Hauff,Michael Reinhardt,Margareta M Mueller,Eva C Woenne,Juergen W Jenne,Mathias Maurer,Guenter W Kauffmann,Wolfhard Semmler,Fabian Kiessling,Moritz Palmowski,Jochen Huppert,Gesa Ladewig,Peter Hauff,Michael Reinhardt,Margareta M Mueller,Eva C Woenne,Juergen W Jenne,Mathias Maurer,Guenter W Kauffmann,Wolfhard Semmler,Fabian Kiessling,

Molecular ultrasound is capable of elucidating the expression of angiogenic markers in vivo. However, the capability of the method for volumetric "multitarget quantification" and for the assessment of antiangiogenic therapy response has rather been investigated. Therefore, we generated cyanoacrylate microbubbles linked to vascular endothelial growth factor receptor 2 (VEGFR2) and alpha(v)beta(3) integrin binding ligands and quantified their accumulation in squamous cell carcinoma xenografts (HaCaT-ras-A-5RT3) in mice with the quantitative volumetric ultrasound scanning technique, sensitive particle acoustic quantification. Specificity of VEGFR2 and alpha(v)beta(3) integrin binding microbubbles was shown, and changes in marker expression during matrix metalloproteinase inhibitor treatment were investigated. In tumors, accumulation of targeted microbubbles was significantly higher compared with nonspecific ones and could be inhibited competitively by addition of the free ligand in excess. Also, multimarker imaging could successfully be done during the same imaging session. Molecular ultrasound further indicated a significant increase of VEGFR2 and alpha(v)beta(3) integrin expression during tumor growth and a considerable decrease in both marker densities after matrix metalloproteinase inhibitor treatment. Histologic data suggested that the increasing VEGFR2 and alpha(v)beta(3) integrin concentrations in tumors during growth are related to an up-regulation of its expression by the endothelial cells, whereas its decrease under therapy is more related to the decreasing relative vessel density. In conclusion, targeted ultrasound appears feasible for the longitudinal molecular profiling of tumor angiogenesis and for the sensitive assessment of therapy effects in vivo. [Mol Cancer Ther 2008;7(1):101-9].

Molecular profiling of angiogenesis with targeted ultrasound imaging: early assessment of antiangiogenic therapy effects. Publishing Authors By Initials

M Palmowski,J Huppert,G Ladewig,P Hauff,M Reinhardt,MM Mueller,EC Woenne,JW Jenne,M Maurer,GW Kauffmann,W Semmler,F Kiessling,M Palmowski,J Huppert,G Ladewig,P Hauff,M Reinhardt,MM Mueller,EC Woenne,JW Jenne,M Maurer,GW Kauffmann,W Semmler,F Kiessling,

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Molecular profiling of angiogenesis with targeted ultrasound imaging: early assessment of antiangiogenic therapy effects. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Molecular cancer therapeutics

VOLUME: 7

Page Numbers: 101-9

Journal Abbreviation: Mol. Cancer Ther.

ISSN: 1535-7163

DAY: 18

MONTH: Jan

YEAR: 2008

Molecular profiling of angiogenesis with targeted ultrasound imaging: early assessment of antiangiogenic therapy effects. Information

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LANGUAGE: eng

NlmUniqueID: 101132535

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AFFILIATION: Junior Group Molecular Imaging, German Cancer Research Center, INF 280, D-69120 Heidelberg, Germany. f.kiessling@dkfz.de.

Country: United States

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MEDLINETA: Mol Cancer Ther

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