Molecular profiles of mitogen activated protein kinase signaling pathways in orofacial development.

Molecular profiles of mitogen activated protein kinase signaling pathways in orofacial development. Research Abstract Details 

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Molecular profiles of mitogen activated protein kinase signaling pathways in orofacial development. Abstract Text:

Saurabh Singh,Xiaolong Yin,M Michele Pisano,Robert M Greene,

BACKGROUND: Formation of the mammalian orofacial region involves multiple signaling pathways regulating sequential expression of and interaction between molecular signals during embryogenesis. The present study examined the expression patterns of members of the MAPK family in developing murine orofacial tissue. METHODS: Total RNA was extracted from developing embryonic orofacial tissue during gestational days (GDs) 12-14 and used to prepare biotinylated cDNA probes, which were then denatured and hybridized to murine MAPK signaling pathways gene arrays. RESULTS: Expression of a number of genes involved in the (ERK1/2) cascade transiently increased in the embryonic orofacial tissue over the developmental period examined. Numerous members of the SAPK/JNK cascade were constitutively expressed in the tissue. Genes known to play a role in p38 MAPK signaling exhibited constitutive expression during orofacial development. Western blot analysis demonstrated that ERK2/1, p38, and SAPK/JNK kinases are present in embryonic orofacial tissue on each of GD 12, 13, and 14. By using phospho-specific antibodies, active ERK was shown to be temporally regulated during orofacial development. Minimal amounts of active p38 and active SAPK/JNK were detected in orofacial tissue during GDs 12-14. CONCLUSIONS: Our study documents specific expression patterns of genes coding for proteins belonging to the ERK1/2, p38, and SAPK/JNK MAPK families in embryonic orofacial tissue. We also demonstrate that active, phosphorylated forms of ERK1/2 only were detected in the embryonic tissue investigated, suggesting a more central role for members of this family in embryonic orofacial development.

Molecular profiles of mitogen activated protein kinase signaling pathways in orofacial development. Publishing Authors By Initials

S Singh,X Yin,MM Pisano,RM Greene,

For similar enzymes and coenzymes: enzymes: transferases: phosphotransferases: phosphotransferases (alcohol group acceptor): protein kinases: protein-serine-threonine kinases: mitogen-activated protein kinases: p38 mitogen-activated protein kinases research abstracts see: enzymes and coenzymes: enzymes: transferases: phosphotransferases: phosphotransferases (alcohol group acceptor): protein kinases: protein-serine-threonine kinases: mitogen-activated protein kinases: p38 mitogen-activated protein kinases research

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Molecular profiles of mitogen activated protein kinase signaling pathways in orofacial development. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Birth defects research. Part A, Clinical and molec

VOLUME: 79

Page Numbers: 35-44

Journal Abbreviation: Birth Defects Res. Part A Clin

ISSN: 1542-0752

DAY: 3

MONTH: Jan

YEAR: 2007

Molecular profiles of mitogen activated protein kinase signaling pathways in orofacial development. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101155107

Molecular profiles of mitogen activated protein kinase signaling pathways in orofacial development. Keywords Mesh Terms:

KEYWORDS: p38 Mitogen-Activated Protein Kinases

MESH TERMS: metabolism

Chemical & Substance for Abstract: Molecular profiles of mitogen activated protein kinase signaling pathways in orofacial development. Information

Substance Name: p38 Mitogen-Activated Protein Kinases

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Molecular profiles of mitogen activated protein kinase signaling pathways in orofacial development.

AFFILIATION: Department of Molecular, Cellular and Craniofacial Biology, University of Louisville Birth Defects Center, ULSD, Louisville, Kentucky 40292, USA.

Country: United States

AGENCY: United States NCRR

GRANT: P20-RR017702

ACRONYM: RR

MEDLINETA: Birth Defects Res A Clin Mol T

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