Molecular probes of DNA bulges: functional assay and spectroscopic analysis.

Molecular probes of DNA bulges: functional assay and spectroscopic analysis. Research Abstract Details 

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Molecular probes of DNA bulges: functional assay and spectroscopic analysis. Abstract Text:

Graham B Jones,Yiqing Lin,Ziwei Xiao,Lizzy Kappen,Irving H Goldberg,

Bulged structures in DNA and RNA have been linked to biomolecular processes involved in numerous diseases, thus probes with affinity for these nucleic acid targets would be of considerable utility to chemical biologists. Herein, we report guided chemical synthesis of small molecules capable of binding to DNA bulges by virtue of their unique (spirocyclic) geometry. The agents, modeled on a natural product congener, show pronounced selectivity for specific bulged motifs and are able to enhance slipped DNA synthesis, a hallmark functional assay of bulge binding. Significantly, bulge-agent complexes demonstrate characteristic fluorescent signatures depending on bulge and flanking sequence in the oligo. It is anticipated that these signature patterns can be harnessed as molecular probes of bulged hotspots in DNA and RNA.

Molecular probes of DNA bulges: functional assay and spectroscopic analysis. Publishing Authors By Initials

GB Jones,Y Lin,Z Xiao,L Kappen,IH Goldberg,

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MEDLINE DATE:

Molecular probes of DNA bulges: functional assay and spectroscopic analysis. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Bioorganic & medicinal chemistry

VOLUME: 15

Page Numbers: 784-90

Journal Abbreviation: Bioorg. Med. Chem.

ISSN: 0968-0896

DAY: 26

MONTH: 10

YEAR: 2006

Molecular probes of DNA bulges: functional assay and spectroscopic analysis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9413298

Molecular probes of DNA bulges: functional assay and spectroscopic analysis. Keywords Mesh Terms:

KEYWORDS: Spectrometry, Mass, Electrospray Ionizat

MESH TERMS: chemistry

Chemical & Substance for Abstract: Molecular probes of DNA bulges: functional assay and spectroscopic analysis. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Molecular probes of DNA bulges: functional assay and spectroscopic analysis.

AFFILIATION: Bioorganic and Medicinal Chemistry Laboratories, Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USA. grjones@neu.edu

Country: England

AGENCY: United States NIGMS

GRANT: R01GM57123

ACRONYM: GM

MEDLINETA: Bioorg Med Chem

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