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Molecular multiplicity of nuclease TT1 from Thermus thermophilus HB8.

Molecular multiplicity of nuclease TT1 from Thermus thermophilus HB8. Research Abstract Details 

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  • Molecular multiplicity of nuclease TT1 from Thermus thermophilus HB8. Abstract Text:

    m takahashiM Takahashi,m kobayashiM Kobayashi,t uchidaT Uchida,

    Purified nuclease TT1 from Thermus thermophilus HB8 has multimolecular weight forms, each of which is composed of three different subunits, alpha (10.8 x 10(4)), beta (7.8 x 10(4)), and gamma (4.1 x 10(4)). The molecular weights of this enzyme were estimated by gel filtration, polyacrylamide gel electrophoresis and equilibrium sedimentation. It was found that most of the enzyme has a molecular weight of about 22 x 10(4) being a monomer having the subunit composition of alpha beta gamma. The remaining part of the enzyme has larger molecular weights and is considered to be size-isomers of alpha beta gamma. The alpha-helical content, 5.5--6.5%, and the beta-structure, about 28%, were estimated from the CD spectrum at 4 degrees C.

    Molecular multiplicity of nuclease TT1 from Thermus thermophilus HB8. Publishing Authors By Initials

    m takahashiM Takahashi,m kobayashiM Kobayashi,t uchidaT Uchida,

    For similar bacteria: gram-negative bacteria: gram-negative aerobic bacteria: gram-negative aerobic rods and cocci: thermus research abstracts see: bacteria: gram-negative bacteria: gram-negative aerobic bacteria: gram-negative aerobic rods and cocci: thermus research

    PUBMED ID PMID:

    MEDLINE DATE:

    Molecular multiplicity of nuclease TT1 from Thermus thermophilus HB8. Journal Published:

    PUBLICATION TYPE: Journal Article

    Journal: Journal of biochemistry

    VOLUME: 90

    Page Numbers: 1521-7

    Journal Abbreviation: J. Biochem.

    ISSN: 0021-924X

    DAY: 19

    MONTH: Nov

    YEAR: 1981

    Molecular multiplicity of nuclease TT1 from Thermus thermophilus HB8. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 376600

    Molecular multiplicity of nuclease TT1 from Thermus thermophilus HB8. Keywords Mesh Terms:

    KEYWORDS: Thermus

    MESH TERMS: enzymology

    Chemical & Substance for Abstract: Molecular multiplicity of nuclease TT1 from Thermus thermophilus HB8. Information

    Substance Name: nuclease TT1

    Registry Number: EC 3.1.15.-

    Grant and Affiliation Information for Molecular multiplicity of nuclease TT1 from Thermus thermophilus HB8.

    AFFILIATION:

    Country: JAPAN

    JAPAN Research PublicationJAPAN Research Publication

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    GRANT:

    ACRONYM:

    MEDLINETA: J Biochem

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