Molecular mechanisms of the biological clock in cultured fibroblasts.

Molecular mechanisms of the biological clock in cultured fibroblasts. Research Abstract Details 

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Molecular mechanisms of the biological clock in cultured fibroblasts. Abstract Text:

K Yagita,F Tamanini,G T van Der Horst,H Okamura,

In mammals, the central circadian pacemaker resides in the hypothalamic suprachiasmatic nucleus (SCN), but circadian oscillators also exist in peripheral tissues. Here, using wild-type and cryptochrome (mCry)-deficient cell lines derived from mCry mutant mice, we show that the peripheral oscillator in cultured fibroblasts is identical to the oscillator in the SCN in (i) temporal expression profiles of all known clock genes, (ii) the phase of the various mRNA rhythms (i.e., antiphase oscillation of Bmal1 and mPer genes), (iii) the delay between maximum mRNA levels and appearance of nuclear mPER1 and mPER2 protein, (iv) the inability to produce oscillations in the absence of functional mCry genes, and (v) the control of period length by mCRY proteins.

Molecular mechanisms of the biological clock in cultured fibroblasts. Publishing Authors By Initials

K Yagita,F Tamanini,GT van Der Horst,H Okamura,

For similar proteins: transcription factors research abstracts see: proteins: transcription factors research

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Molecular mechanisms of the biological clock in cultured fibroblasts. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Science (New York, N.Y.)

VOLUME: 292

Page Numbers: 278-81

Journal Abbreviation: Science

ISSN: 0036-8075

DAY: 13

MONTH: Apr

YEAR: 2001

Molecular mechanisms of the biological clock in cultured fibroblasts. Information

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LANGUAGE: eng

NlmUniqueID: 404511

Molecular mechanisms of the biological clock in cultured fibroblasts. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: Molecular mechanisms of the biological clock in cultured fibroblasts. Information

Substance Name: cryptochrome protein, Drosophila

Registry Number: 0

Grant and Affiliation Information for Molecular mechanisms of the biological clock in cultured fibroblasts.

AFFILIATION: Division of Molecular Brain Science, Department of Brain Sciences, Kobe University Graduate School of Medicine, Chuo-ku, Kobe 650-0017, Japan.

Country: United States

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MEDLINETA: Science

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