Molecular markers for sex determination in papaya ( Carica papaya L.).

Molecular markers for sex determination in papaya ( Carica papaya L.). Research Abstract Details 

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Molecular markers for sex determination in papaya ( Carica papaya L.). Abstract Text:

J C Deputy,R Ming,H Ma,Z Liu,M M M Fitch,M Wang,R Manshardt,J I Stiles,

We have developed molecular markers tightly linked to Sex1, the gene that determines plant sex in papaya ( Carica papaya L.). Three RAPD products have been cloned and a portion of their DNA sequenced. Based on these sequences SCAR primers were synthesized. SCAR T12 and SCAR W11 produce products in hermaphrodite and male plants and only rarely in females. SCAR T1 produces a product in all papayas regardless of plant sex. SCAR T12 and SCAR W11 showed no recombination in a population of 182 F2 plants from a 'SunUp' by 'Kapoho' cross. Based on these results a PCR-based technique for rapidly and accurately determining the sex of papaya plants was developed using either W11 or T12 to detect the hermaphrodite or male allele and T1, which amplifies a product regardless of sex type, as a positive control. The sexing technique, using SCAR T12 and SCAR T1 as a positive control, was used to correctly predict hermaphrodite papaya plants in a population of seedlings with an overall accuracy of 99.2%.

Molecular markers for sex determination in papaya ( Carica papaya L.). Publishing Authors By Initials

JC Deputy,R Ming,H Ma,Z Liu,MM Fitch,M Wang,R Manshardt,JI Stiles,

For similar genetic processes: sex determination (genetics) research abstracts see: genetic processes: sex determination (genetics) research

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Molecular markers for sex determination in papaya ( Carica papaya L.). Journal Published:

PUBLICATION TYPE: Journal Article

Journal: TAG. Theoretical and applied genetics. Theoretisch

VOLUME: 106

Page Numbers: 107-11

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ISSN: 0040-5752

DAY: 12

MONTH: 07

YEAR: 2002

Molecular markers for sex determination in papaya ( Carica papaya L.). Information

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LANGUAGE: eng

NlmUniqueID: 145600

Molecular markers for sex determination in papaya ( Carica papaya L.). Keywords Mesh Terms:

KEYWORDS: Sex Determination (Genetics)

MESH TERMS: genetics

Chemical & Substance for Abstract: Molecular markers for sex determination in papaya ( Carica papaya L.). Information

Substance Name: Genetic Markers

Registry Number: 0

Grant and Affiliation Information for Molecular markers for sex determination in papaya ( Carica papaya L.).

AFFILIATION: Department of Tropical Plant and Soil Sciences, University of Hawaii, Honolulu, HI 96822, USA.

Country: Germany

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MEDLINETA: Theor Appl Genet

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