Molecular level probing of preferential hydration and its modulation by osmolytes through the use of pyranine complexed to hemoglobin.

Molecular level probing of preferential hydration and its modulation by osmolytes through the use of pyranine complexed to hemoglobin. Research Abstract Details 

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Molecular level probing of preferential hydration and its modulation by osmolytes through the use of pyranine complexed to hemoglobin. Abstract Text:

Camille J Roche,Feng Guo,Joel M Friedman,

Two spectroscopic probes are used to expose molecular level changes in hydration shell water interactions that directly relate to such issues as preferential hydration and protein stability. The major focus of the present study is on the use of pyranine (HPT) fluorescence to probe as a function of added osmolytes (PEG, urea, trehalose, and magnesium), the extent to which glycerol is preferentially excluded from the hydration shell of free HPT and HPT localized in the diphosphoglycerate (DPG) binding site of hemoglobin in both solution and in Sol-Gel matrices. The pyranine study is complemented by the use of vibronic side band luminescence from the gadolinium cation that directly exposes the changes in hydrogen bonding between first and second shell waters as a function of added osmolytes. Together the results form the basis for a water partitioning model that can account for both preferential hydration and water/osmolyte-mediated conformational changes in protein structure.

Molecular level probing of preferential hydration and its modulation by osmolytes through the use of pyranine complexed to hemoglobin. Publishing Authors By Initials

CJ Roche,F Guo,JM Friedman,

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Molecular level probing of preferential hydration and its modulation by osmolytes through the use of pyranine complexed to hemoglobin. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of biological chemistry

VOLUME: 281

Page Numbers: 38757-68

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 22

MONTH: 10

YEAR: 2006

Molecular level probing of preferential hydration and its modulation by osmolytes through the use of pyranine complexed to hemoglobin. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Molecular level probing of preferential hydration and its modulation by osmolytes through the use of pyranine complexed to hemoglobin. Keywords Mesh Terms:

KEYWORDS: Water

MESH TERMS: chemistry

Chemical & Substance for Abstract: Molecular level probing of preferential hydration and its modulation by osmolytes through the use of pyranine complexed to hemoglobin. Information

Substance Name: Trehalose

Registry Number: 99-20-7

Grant and Affiliation Information for Molecular level probing of preferential hydration and its modulation by osmolytes through the use of pyranine complexed to hemoglobin.

AFFILIATION: Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

Country: United States

AGENCY: United States NIAID

GRANT: R01 AI 052258

ACRONYM: AI

MEDLINETA: J Biol Chem

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