[Molecular identification of human/goat xenogeneic model]

[Molecular identification of human/goat xenogeneic model] Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

[Molecular identification of human/goat xenogeneic model] Abstract Text:

Meijue Chen,Jingbin Yan,Yudan Fang,Zhaorui Ren,Yanping Xiao,Shuzhen Huang,

OBJECTIVE: To identify the human hematopoietic stem cells from the human/goat xenogeneic model with molecular techniques. METHODS: DNA and total RNA were extracted from 11 transplanted goat peripheral blood cells. Human CD(34), GPA and SRY genes were amplified with PCR in these samples, and CD(34), GPA mRNA transcripts were detected using RT-PCR in 5 and 6 goat peripheral blood cells, respectively. Southern blot analysis was performed in 8 goat DNAs to detect the human specific alpha-satellite sequence. Meanwhile FISH was also performed to detect the human cells in goat blood with a probe of human Y chromosome. RESULTS: Human CD(34) and GPA genes could be detected with PCR in all the 11 goats, and SRY gene did in 5 goats transplanted with hematopoietic stem cells derived from male human babies. Southern blot showed that human specific alpha-satellite sequence was present in 8 goats. By RT-PCR, human CD(34) mRNA was detected in 5 experimental goats, GPA mRNA was found in the other 6 experimental goats and FISH assay showed that some peripheral blood cells of the human/goat xenogeneic model were positive. CONCLUSION: Existence of human cells in the recipient goats was identified by molecular detection, which was feasible for the examination of human/goat xenogeneic models.

[Molecular identification of human/goat xenogeneic model] Publishing Authors By Initials

M Chen,J Yan,Y Fang,Z Ren,Y Xiao,S Huang,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

[Molecular identification of human/goat xenogeneic model] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zhonghua xue ye xue za zhi = Zhonghua xueyexue zaz

VOLUME: 23

Page Numbers: 634-7

Journal Abbreviation: Zhonghua Xue Ye Xue Za Zhi

ISSN: 0253-2727

DAY: 1

MONTH: Dec

YEAR: 2002

[Molecular identification of human/goat xenogeneic model] Information

Number of References:

LANGUAGE: chi

NlmUniqueID: 8212398

[Molecular identification of human/goat xenogeneic model] Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: [Molecular identification of human/goat xenogeneic model] Information

Substance Name:

Registry Number:

Grant and Affiliation Information for [Molecular identification of human/goat xenogeneic model]

AFFILIATION: Shanghai Institute of Medical Genetics, Shanghai Children Hospital, Shanghai 200040, China.

Country: China

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Zhonghua Xue Ye Xue Za Zhi

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Molecular identification of human/goat xenogeneic model Related Publications

• Clinicopathological observation of renal adenomas.
• Effects of the central amygdaloid nucleus lesions on the gustatory responses in the parabrachial nucleus in rats.
• Ehlers-Danlos syndrome: case and pedigree report and review.
• Telangiectatic osteosarcoma: a clinicopathologic analysis of 14 cases.
• [A rapid and simple detection of DNA fragment with point mutation by capillary electrophoresis]
• [A simple and rapid new method for SNP typing by single-tube bi-directional allele specific amplification]
• [Algorithms of multiband remote sensing for coastal red tide waters]
• [Analysis of factors correlated to death and reinfarction in patients with acute myocardial infarction]
• [Application of liposuction in scar repair]
• [Biomechanical evaluation of the stability of General spine system]
• [Clinical analysis of combined resection for T4 gastric cancer: report of 69 cases]
• [Determination of residues of organic chloride pesticide in Chinese Medicinal materials with capillary gas chromatography]
• [Ecological characteristics of Prorocentrum dentatum and the cause of harmful algal bloom formation in China Sea]
• [Effect of intracerebroventricular injection of histamine on carotid sinus baroreceptor reflex in anesthetized rats and its mechanism]
• [Experience with cardioventer defibrillator therapy in 113 patients]
• [Lower back pain in truck drivers working in plateau areas and its prevention]
• [Mammographic findings of gynecomastia]
• [Miniature quadrupole mass spectrometer array and its applications in manned spaceflight]
• [Molecular identification of human/goat xenogeneic model]
• [Phosphorylated JNK mediated apoptosis induced by all trans retinoid acid in human retinoblastoma cell line]
• [Protective effects of c-jun anti-sense gene recombinant transfection on rat cardiomyocytes inflicted by hypoxia and burn serum]
• [Purification and stability studies of prokaryotic PDCD5 protein]
• [Rat heart transplantation immune tolerance induced by blockade of B7-CD28 interaction]
• [Repair of facial defect with expanded skin flap from medial upper arm]
• [Secondary reconstruction of post-fracture deformities in the nasal-orbital ethmoid region]
• [Study on morphological change in intestinal mucosa from injury to repair after hemorrhagic shock]
• [Study on the therapeutic effect of combined use of obidoxime and atropine with respiratory machine on respiratory muscle paralysis caused by omethoate poisoning of rats]
• [The correlations between glucocorticoid receptor of lymphocyte and immune function]
• [The reason and prevention of upper cervical reoperations]
• [The relation and evaluation of the vertebral artery and the nerve root to posterior lateral mass screw fixation]