Molecular genetic probing of energy coupling by the yeast plasma membrane proton pump.

Molecular genetic probing of energy coupling by the yeast plasma membrane proton pump. Research Abstract Details 

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Molecular genetic probing of energy coupling by the yeast plasma membrane proton pump. Abstract Text:

P Soteropoulos,G Wang,D S Perlin,

Genetic probing of PMA1, which encodes the plasma membrane H(+)-ATPase, has highlighted the putative role of the N-terminal half of the enzyme in the coupling process. Recent second-site suppressor studies indicate that significant interactions occur between the region near the site of phosphorylation, stalk segment 3 (S3), and the N-terminal transmembrane segments. Saturation mutagenesis was used to explore I183 in S2, which partially uncouples proton transport when converted to alanine. Numerous substitutions could be made at this position. However, stable substitutions with Arg, Tyr or Asn were often accompanied by second-site mutations at the extreme C-terminus, suggesting a close interaction between these regions. Several mutations in the putative stalk domain are known to alter coupling, and scanning glycine and proline mutagenesis was used to probe the predicted alpha-helical character of the stalk segments. The results indicate that the introduction of proline or glycine in S2, S4 or S5, was highly disruptive to enzyme function often resulting in cell death. Similar substitutions in stalk 3 yielded viable but significantly altered enzymes. These results suggest that the helical properties of these segments may be important for catalysis. Finally, the stalk region has been modeled as a helical bundle, which helps account for the effects of specific perturbations in this region.

Molecular genetic probing of energy coupling by the yeast plasma membrane proton pump. Publishing Authors By Initials

P Soteropoulos,G Wang,DS Perlin,

For similar fungi: yeasts research abstracts see: fungi: yeasts research

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Molecular genetic probing of energy coupling by the yeast plasma membrane proton pump. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Acta physiologica Scandinavica. Supplementum

VOLUME: 643

Page Numbers: 115-22

Journal Abbreviation:

ISSN: 0302-2994

DAY: 20

MONTH: Aug

YEAR: 1998

Molecular genetic probing of energy coupling by the yeast plasma membrane proton pump. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376307

Molecular genetic probing of energy coupling by the yeast plasma membrane proton pump. Keywords Mesh Terms:

KEYWORDS: Yeasts

MESH TERMS: enzymology

Chemical & Substance for Abstract: Molecular genetic probing of energy coupling by the yeast plasma membrane proton pump. Information

Substance Name: Adenosine Triphosphate

Registry Number: 56-65-5

Grant and Affiliation Information for Molecular genetic probing of energy coupling by the yeast plasma membrane proton pump.

AFFILIATION: Public Health Research Institute, New York, NY 10016, USA.

Country: ENGLAND

AGENCY: United States NIGMS

GRANT: GM 38225

ACRONYM: GM

MEDLINETA: Acta Physiol Scand Suppl

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