Molecular enzymology of the glyoxalase system.

Molecular enzymology of the glyoxalase system. Research Abstract Details 

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Molecular enzymology of the glyoxalase system. Abstract Text:

The glyoxalase system catalyzes the conversion of 2-oxoaldehydes into the corresponding 2-hydroxyacids. This biotransformation involves two separate enzymes, glyoxalase I and glyoxalase II, which bring about two consecutive reactions involving the thiol-containing tripeptide glutathione as a cofactor. The physiologically most important substrate methylglyoxal is converted by glyoxalase I into S-D-lactoyl-glutathione in the first reaction. Subsequently, glyoxalase II catalyzes the hydrolysis of this thiolester into D-lactic acid and free glutathione. The structures of both enzymes have been obtained via molecular cloning, heterologous expression, and X-ray diffraction analysis. Glyoxalase I and glyoxalase II are metalloenzymes and zinc plays an essential role in their diverse catalytic mechanisms. Both enzymes appear linked to a variety of pathological conditions, but further investigations are required to clarify the different physiological aspects of the glyoxalase system.

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Molecular enzymology of the glyoxalase system. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Drug metabolism and drug interactions

VOLUME: 23

Page Numbers: 13-27

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ISSN: 0792-5077

DAY: 6

MONTH: 06

YEAR: 2008

Molecular enzymology of the glyoxalase system. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Molecular enzymology of the glyoxalase system.

AFFILIATION: Department of Biochemistry and Organic Chemistry, Uppsala University, Biomedical Center, Uppsala, Sweden. Bengt.Mannervik@biorg.uu.se

Country: England

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MEDLINETA: Drug Metabol Drug Interact

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