Molecular cloning of a cDNA encoding rat NADH-cytochrome b5 reductase and the corresponding gene.

Molecular cloning of a cDNA encoding rat NADH-cytochrome b5 reductase and the corresponding gene. Research Abstract Details 

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Molecular cloning of a cDNA encoding rat NADH-cytochrome b5 reductase and the corresponding gene. Abstract Text:

S Zenno,M Hattori,Y Misumi,T Yubisui,Y Sakaki,

Rat cDNA encoding NADH-cytochrome b5 reductase (b5R) was isolated from a rat liver cDNA library using a human b5R cDNA as a probe. The cDNA was 1,905 nucleotides long, consisting of a 5'-terminal untranslated region of 38 nucleotides long, an open reading frame region of 903 nucleotides long encoding 301 amino acid residues, a 3'-terminal untranslated region of 952 nucleotide long, and a poly(A) tail. The amino acid sequence deduced from the cDNA sequence indicated that the rat b5R precursor contained only one extra amino acid (Met) residue at the N terminus, in comparison with the mature form of the enzyme, suggesting that no extra leader peptide is required for translocation of the enzyme to the microsome membrane. Genomic DNA encoding the b5R gene was isolated from rat genomic DNA libraries. The gene was about 17 kb long, and consisted of nine exons and eight introns. The junction between the membrane-binding and catalytic domains of the enzyme was found in the middle of exon 2, suggesting the possibility that the two forms of the enzyme, namely the membrane-bound and soluble forms, are generated through post-translational processing. The possible promoter region of the gene contained no TATA box but four GC box sequences (GGGCGG and CCGCCC), representing potential binding sites for the transcription factor, SP1. The b5R gene seems to have structural characteristics of a house-keeping gene.

Molecular cloning of a cDNA encoding rat NADH-cytochrome b5 reductase and the corresponding gene. Publishing Authors By Initials

S Zenno,M Hattori,Y Misumi,T Yubisui,Y Sakaki,

For similar animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats research abstracts see: animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats research

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Molecular cloning of a cDNA encoding rat NADH-cytochrome b5 reductase and the corresponding gene. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 107

Page Numbers: 810-6

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 1990

Molecular cloning of a cDNA encoding rat NADH-cytochrome b5 reductase and the corresponding gene. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Molecular cloning of a cDNA encoding rat NADH-cytochrome b5 reductase and the corresponding gene. Keywords Mesh Terms:

KEYWORDS: Rats

MESH TERMS: genetics

Chemical & Substance for Abstract: Molecular cloning of a cDNA encoding rat NADH-cytochrome b5 reductase and the corresponding gene. Information

Substance Name: Cytochrome-B(5) Reductase

Registry Number: EC 1.6.2.2

Grant and Affiliation Information for Molecular cloning of a cDNA encoding rat NADH-cytochrome b5 reductase and the corresponding gene.

AFFILIATION: Research Laboratory for Genetic Information, Kyushu University, Fukuoka.

Country: JAPAN

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MEDLINETA: J Biochem

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ACCESSION NUMBER: D00718

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