Molecular cloning of a brain-specific, developmentally regulated neuregulin 1 (NRG1) isoform and identification of a functional promoter variant associated with schizophrenia.

Molecular cloning of a brain-specific, developmentally regulated neuregulin 1 (NRG1) isoform and identification of a functional promoter variant associated with schizophrenia. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Molecular cloning of a brain-specific, developmentally regulated neuregulin 1 (NRG1) isoform and identification of a functional promoter variant associated with schizophrenia. Abstract Text:

Wei Tan,Yanhong Wang,Bert Gold,Jingshan Chen,Michael Dean,Paul J Harrison,Daniel R Weinberger,Amanda J Law,

Neuregulin 1 (NRG1) is essential for the development and function of multiple organ systems, and its dysregulation has been linked to diseases such as cancer and schizophrenia. Recently, altered expression of a novel isoform (type IV) in the brain has been associated with schizophrenia-related genetic variants, especially rs6994992 (SNP8NRG243177). Here we have isolated and characterized full-length NRG1 type IV cDNAs from the adult and fetal human brain and identified novel splice variants of NRG1. Full-length type IV spans 1.8 kb and encodes a putative protein of 590 amino acids with a predicted molecular mass of approximately 66 kDa. The transcript consists of 11 exons with an Ig-like domain, an epidermal growth factor-like (EGF) domain, a beta-stalk, a transmembrane domain, and a cytoplasmic "a-tail," placing it in the beta1a NRG1 subclass. NRG1 type IV was not detected in any tissues except brain and a putative type IV NRG1 protein of 66 kDa was similarly brain-specific. Type IV transcripts are more abundantly expressed in the fetal brain, where, in addition to the full-length structure, two novel type IV variants were identified. In vitro luciferase-reporter assays demonstrate that the 5' promoter region upstream of type IV is functional, with differential activity associated with genetic variation at rs6994992, and that promoter competition may impact on type IV expression. Our data suggest that type IV is a unique brain-specific NRG1 that is differentially expressed and processed during early development, is translated, and its expression regulated by a schizophrenia risk-associated functional promoter or single nucleotide polymorphism (SNP).

Molecular cloning of a brain-specific, developmentally regulated neuregulin 1 (NRG1) isoform and identification of a functional promoter variant associated with schizophrenia. Publishing Authors By Initials

W Tan,Y Wang,B Gold,J Chen,M Dean,PJ Harrison,DR Weinberger,AJ Law,

For similar mental disorders: schizophrenia and disorders with psychotic features: schizophrenia research abstracts see: mental disorders: schizophrenia and disorders with psychotic features: schizophrenia research

PUBMED ID PMID:

MEDLINE DATE:

Molecular cloning of a brain-specific, developmentally regulated neuregulin 1 (NRG1) isoform and identification of a functional promoter variant associated with schizophrenia. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 24343-51

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 12

MONTH: 06

YEAR: 2007

Molecular cloning of a brain-specific, developmentally regulated neuregulin 1 (NRG1) isoform and identification of a functional promoter variant associated with schizophrenia. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Molecular cloning of a brain-specific, developmentally regulated neuregulin 1 (NRG1) isoform and identification of a functional promoter variant associated with schizophrenia. Keywords Mesh Terms:

KEYWORDS: Schizophrenia

MESH TERMS: genetics

Chemical & Substance for Abstract: Molecular cloning of a brain-specific, developmentally regulated neuregulin 1 (NRG1) isoform and identification of a functional promoter variant associated with schizophrenia. Information

Substance Name: Protein Isoforms

Registry Number: 0

Grant and Affiliation Information for Molecular cloning of a brain-specific, developmentally regulated neuregulin 1 (NRG1) isoform and identification of a functional promoter variant associated with schizophrenia.

AFFILIATION: SAIC-Frederick, NCI, National Institutes of Health, Frederick, Maryland 21702, USA.

Country: United States

AGENCY: United States NCI

GRANT: N01-CO-12400

ACRONYM: CO

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Molecular cloning of a brain-specific, developmentally regulated neuregulin 1 NRG1 isoform and identification of a functional promoter variant associated with schizophrenia Related Publications

• -Arrestin-Dependent {micro}-Opioid Receptor-Activated Extracellular Signal-Regulated Kinases (ERKs) Translocate to Nucleus in Contrast to G Protein-Dependent ERK Activation.
• A firm persuasion in our work. Occupational therapy: a journey driven by curiosity.
• Aedes aegypti ferritin heavy chain homologue: feeding of iron or blood influences message levels, lengths and subunit abundance.
• Agonist-dependent postsynaptic effects of opioids on miniature excitatory postsynaptic currents in cultured hippocampal neurons.
• An assessment of chemical pollution from the MSC Napoli.
• An attempt to demonstrate neutralizing antibodies to the mammary tumour milk agent in mice.
• Deep proteome profiling of sera from never-smoked lung cancer patients.
• Differentiating intracranial aspergillosis from a high grade glioma using MRI and MR spectroscopic imaging.
• Distinct effects of individual opioids on the morphology of spines depend upon the internalization of mu opioid receptors.
• Fluid transport across the isolated porcine ciliary epithelium.
• Gs15p is there a better way to determine private surgical fees in new zealand?
• Interview therapy of psychosomatic arthritis.
• Kinematic condition for multicomponent lattice Boltzmann simulation.
• Modelling the hepatitis C virus epidemic in Australia.
• Modelling the population-level impact of vaccination on the transmission of human papillomavirus type 16 in Australia.
• Molecular cloning of a brain-specific, developmentally regulated neuregulin 1 (NRG1) isoform and identification of a functional promoter variant associated with schizophrenia.
• Nonparaxial propagation of a cylindrical beam with Lanczos reduction.
• Novel fatty acids from the royal jelly of honeybees (Apis mellifera, L.).
• Osteotomy of the spine in the treatment of severe dorsal kyphosis; four cases.
• Phosphorylation of the delta-opioid receptor regulates its beta-arrestins selectivity and subsequent receptor internalization and adenylyl cyclase desensitization.
• Propagation of nonparaxial beams with a modified Arnoldi method.
• Ring location in cyclopropane fatty acid esters by a mass spectrometric method.
• Self-reported psychological States and physiological responses to different types of motivational general imagery.
• Some notes on hyperphoria with illustrative case histories.
• The hygiene of the scalp and hair.
• The importance of size and growth in infancy: integrated findings from systematic reviews of scientific evidence and lay perspectives.
• The role of a clinical director in developing an innovative assertive community treatment team targeting ethno-racial minority patients.
• The toxicological impacts of oil and chemically dispersed oil: UV mediated phototoxicity and implications for environmental effects, statutory testing and response strategies.
• Use of chewing gum in reducing postoperative ileus after elective colorectal resection: a systematic review.
• Use of colonic stents in emergent malignant left colonic obstruction: a markov chain monte carlo decision analysis.