Molecular cloning, expression pattern and comparative analysis of chitin synthase gene B in Spodoptera exigua.

Molecular cloning, expression pattern and comparative analysis of chitin synthase gene B in Spodoptera exigua. Research Abstract Details 

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Molecular cloning, expression pattern and comparative analysis of chitin synthase gene B in Spodoptera exigua. Abstract Text:

N Senthil Kumar,Bin Tang,Xiaofei Chen,Honggang Tian,Wenqing Zhang,

The chitin synthase (CHS) gene B (4781 bp) of Spodoptera exigua (SeCHSB) was cloned by reverse-transcription PCR (RT-PCR) and 3'/5' RACE from the midgut. SeCHSB contains an open reading frame of 4572 nucleotides, encoding a protein of 1523 amino acids with a predicted molecular mass of approximately 174.6 kDa. Alignment of SeCHSB with class B CHSs of other insects showed a high degree of conservation in the putative catalytic domain region. The structure of the SeCHSB gene was analyzed and was found to be the same as that of Manduca sexta CHSB (MsCHSB), including 23 exons and 22 introns but without alternative exons. Southern blot analysis revealed that SeCHSB was a single copy gene and the presence of only two chitin synthase genes in S. exigua. Further investigation indicated that SeCHSB was specifically expressed in the midgut, and its transcript existed constitutively in the midgut from the 3rd instar larval stage to prepupae and reached highest expression on the 1st day of the fifth instar larval stage. These data suggest that SeCHSB is very important in midgut formation and development. Chitin synthase gene comparisons between different classes of insects using software tools revealed some interesting aspects of the similarity and divergence of the gene in the Class Insecta.

Molecular cloning, expression pattern and comparative analysis of chitin synthase gene B in Spodoptera exigua. Publishing Authors By Initials

NS Kumar,B Tang,X Chen,H Tian,W Zhang,

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Molecular cloning, expression pattern and comparative analysis of chitin synthase gene B in Spodoptera exigua. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Comparative biochemistry and physiology. Part B, B

VOLUME: 149

Page Numbers: 447-53

Journal Abbreviation: Comp. Biochem. Physiol. B, Bio

ISSN: 1096-4959

DAY: 28

MONTH: 11

YEAR: 2007

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LANGUAGE: eng

NlmUniqueID: 9516061

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Grant and Affiliation Information for Molecular cloning, expression pattern and comparative analysis of chitin synthase gene B in Spodoptera exigua.

AFFILIATION: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.

Country: England

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MEDLINETA: Comp Biochem Physiol B Biochem

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