Molecular cloning, expression and properties of an alpha/beta-Galactoside alpha2,3-sialyltransferase from Vibrio sp. JT-FAJ-16.

Molecular cloning, expression and properties of an alpha/beta-Galactoside alpha2,3-sialyltransferase from Vibrio sp. JT-FAJ-16. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Molecular cloning, expression and properties of an alpha/beta-Galactoside alpha2,3-sialyltransferase from Vibrio sp. JT-FAJ-16. Abstract Text:

Yoshimitsu Takakura,Hiroshi Tsukamoto,Takeshi Yamamoto,Yoshimitsu Takakura,Hiroshi Tsukamoto,Takeshi Yamamoto,

We cloned, expressed and characterized a novel alpha/beta-galactoside alpha2,3-sialyltransferase from Vibrio sp. bacterium JT-FAJ-16. Using a alpha2,3-sialyltransferase gene from a marine bacterium as a probe, a DNA sequence encoding a 402-amino-acid protein was identified from the JT-FAJ-16 genomic library. The protein showed 27.3-64.7% identity to the bacterial sialyltransferases classified into glycosyltransferase family 80. The protein showed sialyltransferase activity when expressed in Escherichia coli. The N-terminal truncated form of the enzyme was amplified in E. coli and its recovered activity was 215.7 unit/l culture medium. It was purified as a single band on SDS-PAGE through the three chromatographic steps. The specific activity of the purified recombinant enzyme reached 57.5 unit/mg protein. The alpha2,3sialylation was confirmed by (1)H- and (13)C-NMR analyses of the reaction products. The enzyme was optimally active at pH 5.5 and at 20 degrees C. Interestingly, the enzyme used both the alpha- and beta-anomers of galactosides as acceptors, suggesting that it can be described as an alpha/beta-galactoside alpha2,3-sialyltransferase. The enzyme had a wide range of acceptor substrate specificities. It transferred N-acetylneuraminic acid (NeuAc) to various monosaccharides and various oligosaccharides, and both N-linked and O-linked asialo-glycoprotein. These results suggest that the enzyme can be used as a powerful tool for the study for glycotechnology.

Molecular cloning, expression and properties of an alpha/beta-Galactoside alpha2,3-sialyltransferase from Vibrio sp. JT-FAJ-16. Publishing Authors By Initials

Y Takakura,H Tsukamoto,T Yamamoto,Y Takakura,H Tsukamoto,T Yamamoto,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Molecular cloning, expression and properties of an alpha/beta-Galactoside alpha2,3-sialyltransferase from Vibrio sp. JT-FAJ-16. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 142

Page Numbers: 403-12

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 2

MONTH: 08

YEAR: 2007

Molecular cloning, expression and properties of an alpha/beta-Galactoside alpha2,3-sialyltransferase from Vibrio sp. JT-FAJ-16. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Molecular cloning, expression and properties of an alpha/beta-Galactoside alpha2,3-sialyltransferase from Vibrio sp. JT-FAJ-16. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Molecular cloning, expression and properties of an alpha/beta-Galactoside alpha2,3-sialyltransferase from Vibrio sp. JT-FAJ-16. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Molecular cloning, expression and properties of an alpha/beta-Galactoside alpha2,3-sialyltransferase from Vibrio sp. JT-FAJ-16.

AFFILIATION: Glycotechnology Business Unit, Japan Tobacco Inc, 700 Higashibara, Iwata, Shizuoka, Japan. yoshimitsu.takakura@ims.jti.co.jp

Country: Japan

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem (Tokyo)

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Molecular cloning, expression and properties of an alpha/beta-Galactoside alpha2,3-sialyltransferase from Vibrio sp JT-FAJ-16 Related Publications

• CO2-enhanced sonographically guided radiofrequency ablation and transcatheter arterial chemoembolization for small hepatocellular carcinoma poorly defined on conventional sonography.
• Contested dominance modifies the anovulatory consequences of social subordination in female marmosets.
• Decreased fractal correlation in diurnal physical activity in chronic fatigue syndrome.
• Descriptive study of gallbladder, extrahepatic bile duct, and ampullary cancers in the United States, 1997-2002.
• Diastereo- and enantioselective synthesis of nitroso Diels-Alder-type bicycloketones using dienamine: mechanistic insight into sequential nitroso Aldol/Michael reaction and application for optically pure 1-amino-3,4-diol synthesis.
• Drosophila CTLA-2-like protein (D/CTLA-2) inhibits cysteine proteinase 1 (CP1), a cathepsin L-like enzyme.
• Dynamic changes in vesicular glutamate transporter 1 function and expression related to methamphetamine-induced glutamate release.
• Effects of bilateral jugular vein ligation on local cerebral blood flow.
• Effects of brain lesions on taste-potentiated odor aversion in rats.
• Effects of chronic unpredictable stress and methamphetamine on hippocampal glutamate function.
• Evaluation of osteogenic/chondrogenic cellular proliferation and differentiation in the xenogeneic periosteal graft.
• Gene expression signatures that can discriminate oral leukoplakia subtypes and squamous cell carcinoma.
• Haloperidol treatment after high-dose methamphetamine administration is excitotoxic to GABA cells in the substantia nigra pars reticulata.
• Imaging of Regional Differences of Muscle Oxygenation during Exercise Using Spatially Resolved NIRS.
• Interactions between methamphetamine and environmental stress: role of oxidative stress, glutamate and mitochondrial dysfunction.
• Intraoperative electro-oculographic Monitoring for Skull Base Surgery.
• Involvement of the Mesolimbic System in Palatability-induced Ingestion.
• Ketamine and N-acetylaspartylglutamate peptidase inhibitor exert analgesia in bone cancer pain.
• Kinetic mechanism of the fastest motor protein, Chara myosin.
• Loss of the TOR kinase Tor2 mimics nitrogen starvation and activates the sexual development pathway in fission yeast.
• Menatetrenone, a vitamin K2 analogue, inhibits hepatocellular carcinoma cell growth by suppressing cyclin D1 expression through inhibition of nuclear factor kappaB activation.
• Myostatin modulates adipogenesis to generate adipocytes with favorable metabolic effects.
• Negative regulation of BMP/Smad signaling by Tob in osteoblasts.
• Prior exposure to chronic stress and MDMA potentiates mesoaccumbens dopamine release mediated by the 5-HT(1B) receptor.
• Regulation of Wnt signalling by receptor-mediated endocytosis.
• Simultaneous gene transfer of bone morphogenetic protein (BMP) -2 and BMP-7 by in vivo electroporation induces rapid bone formation and BMP-4 expression.
• The Role of Orexigenic Neuropeptides in the Ingestion of Sweet-tasting Substances in Rats.
• The relationship between cerebral blood flow and cognitive function in patients with brain insult of various etiology.
• The use of polylactic acid/polyglycolic acid copolymer and gelatin sponge complex containing human recombinant bone morphogenetic protein-2 following condylectomy in rabbits.
• Trehalose Sensitivity of the Gustatory Receptor Neurons Expressing Wild-type, Mutant and Ectopic Gr5a in Drosophila.