Molecular cloning and sequencing of plasma membrane H(+)-ATPase gene from the salt-tolerant yeast Zygosaccharomyces rouxii.

Molecular cloning and sequencing of plasma membrane H(+)-ATPase gene from the salt-tolerant yeast Zygosaccharomyces rouxii. Research Abstract Details 

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Molecular cloning and sequencing of plasma membrane H(+)-ATPase gene from the salt-tolerant yeast Zygosaccharomyces rouxii. Abstract Text:

Y Watanabe,M Shiramizu,Y Tamai,

A plasma membrane H(+)-ATPase gene from the salt-tolerant yeast Zygosaccharomyces rouxii was isolated by probing the genomic DNA library with a labeled DNA fragment derived from the Saccharomyces cerevisiae H(+)-ATPase gene (PMA1) and the nucleotide sequence of a cloned gene was determined. The gene encoded a polypeptide of molecular weight 100,060 that consisted of 920 amino acid residues. The deduced amino acid sequence was 83% homologous to that of S. cerevisiae H(+)-ATPase and was highly similar to the conserved sequences in the plasma membrane ATPase family comprising yeast plasma membrane H(+)-ATPases. The peptide motifs involved in the ATPase functions were found in the Z. rouxii H(+)-ATPase sequence. The result of Northern analysis indicated that the size of the transcript of the Z. rouxii H(+)-ATPase gene was the same as that of S. cerevisiae PMA1.

Molecular cloning and sequencing of plasma membrane H(+)-ATPase gene from the salt-tolerant yeast Zygosaccharomyces rouxii. Publishing Authors By Initials

Y Watanabe,M Shiramizu,Y Tamai,

For similar fungi: yeasts research abstracts see: fungi: yeasts research

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Molecular cloning and sequencing of plasma membrane H(+)-ATPase gene from the salt-tolerant yeast Zygosaccharomyces rouxii. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 110

Page Numbers: 237-40

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1991

Molecular cloning and sequencing of plasma membrane H(+)-ATPase gene from the salt-tolerant yeast Zygosaccharomyces rouxii. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Molecular cloning and sequencing of plasma membrane H(+)-ATPase gene from the salt-tolerant yeast Zygosaccharomyces rouxii. Keywords Mesh Terms:

KEYWORDS: Yeasts

MESH TERMS: enzymology

Chemical & Substance for Abstract: Molecular cloning and sequencing of plasma membrane H(+)-ATPase gene from the salt-tolerant yeast Zygosaccharomyces rouxii. Information

Substance Name: Proton-Translocating ATPases

Registry Number: EC 3.6.3.14

Grant and Affiliation Information for Molecular cloning and sequencing of plasma membrane H(+)-ATPase gene from the salt-tolerant yeast Zygosaccharomyces rouxii.

AFFILIATION: Department of Bioresources, Faculty of Agriculture, Ehime University.

Country: JAPAN

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MEDLINETA: J Biochem

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