A cDNA clone specific for rat ribosomal protein S26 was isolated by a positive hybridization translation assay from a cDNA library made for 8-9S poly(A)mRNA from regenerating rat liver. The nucleotide sequence of the cDNA was determined. The sequence contains 23 base pairs in the 5' noncoding region, 345 base pairs in the protein coding region and 67 base pairs in the 3' noncoding region besides the poly(A) tail. The primary structure of the protein S26 was deduced from the nucleotide sequence. It consists of 115 amino acids. Its molecular weight is 13,015 and its pI is about 11.1. The calculated amino acid composition is consistent with the reported composition of S26. From the results of Southern blot analysis, the protein S26 appears to have multiple genes.
Molecular cloning and nucleotide sequence of DNA complementary to rat ribosomal protein S26 messenger RNA. Publishing Authors By Initials
Molecular cloning and nucleotide sequence of DNA complementary to rat ribosomal protein S26 messenger RNA. Journal Published:
PUBLICATION TYPE: Research Support, Non-U.S. Gov
Journal: Journal of biochemistry
VOLUME: 97
Page Numbers: 983-92
Journal Abbreviation: J. Biochem.
ISSN: 0021-924X
DAY: 19
MONTH: Apr
YEAR: 1985
Molecular cloning and nucleotide sequence of DNA complementary to rat ribosomal protein S26 messenger RNA. Information
Number of References:
LANGUAGE: eng
NlmUniqueID: 376600
Molecular cloning and nucleotide sequence of DNA complementary to rat ribosomal protein S26 messenger RNA. Keywords Mesh Terms:
KEYWORDS: Ribosomal Proteins
MESH TERMS: genetics
Chemical & Substance for Abstract: Molecular cloning and nucleotide sequence of DNA complementary to rat ribosomal protein S26 messenger RNA. Information
Substance Name: DNA Restriction Enzymes
Registry Number: EC 3.1.21.-
Grant and Affiliation Information for Molecular cloning and nucleotide sequence of DNA complementary to rat ribosomal protein S26 messenger RNA.
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Country: JAPAN
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MEDLINETA: J Biochem
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ACCESSION NUMBER: X02414
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