Molecular cloning and nucleotide sequence analysis of the maltose-inducible porin gene of Aeromonas salmonicida.

Molecular cloning and nucleotide sequence analysis of the maltose-inducible porin gene of Aeromonas salmonicida. Research Abstract Details 

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Molecular cloning and nucleotide sequence analysis of the maltose-inducible porin gene of Aeromonas salmonicida. Abstract Text:

S J Dodsworth,A J Bennett,G Coleman,

The gene for the Aeromonas salmonicida maltose-inducible porin (maltoporin) was cloned into phagemid pTZ18R in two restriction fragments, 0.6-kb PstI/KpnI and 1.7-kb SphI, of genomic DNA and their nucleotide sequences were determined. Open reading frames of 1329 and 1335 bp translated into sequences of 443 and 445 amino acids, with a 23 or 25 amino acid signal sequence and a 420 amino acid mature protein of molecular mass 46424 Da. Putative ribosome binding sites, AGGA and GGGAA, occurred 9 bp upstream of two possible ATG initiation codons. The A. salmonicida gene product showed a high degree of similarity with Escherichia coli LamB, and codon usage was very similar to that of another A. salmonicida outer membrane protein but markedly different from those of extracellular proteins.

Molecular cloning and nucleotide sequence analysis of the maltose-inducible porin gene of Aeromonas salmonicida. Publishing Authors By Initials

SJ Dodsworth,AJ Bennett,G Coleman,

For similar proteins: membrane proteins: receptors, cell surface: receptors, virus research abstracts see: proteins: membrane proteins: receptors, cell surface: receptors, virus research

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Molecular cloning and nucleotide sequence analysis of the maltose-inducible porin gene of Aeromonas salmonicida. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: FEMS microbiology letters

VOLUME: 112

Page Numbers: 191-7

Journal Abbreviation: FEMS Microbiol. Lett.

ISSN: 0378-1097

DAY: 1

MONTH: Sep

YEAR: 1993

Molecular cloning and nucleotide sequence analysis of the maltose-inducible porin gene of Aeromonas salmonicida. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7705721

Molecular cloning and nucleotide sequence analysis of the maltose-inducible porin gene of Aeromonas salmonicida. Keywords Mesh Terms:

KEYWORDS: Receptors, Virus

MESH TERMS: genetics

Chemical & Substance for Abstract: Molecular cloning and nucleotide sequence analysis of the maltose-inducible porin gene of Aeromonas salmonicida. Information

Substance Name: Maltose

Registry Number: 69-79-4

Grant and Affiliation Information for Molecular cloning and nucleotide sequence analysis of the maltose-inducible porin gene of Aeromonas salmonicida.

AFFILIATION: Department of Biochemistry, Nottingham University Medical School, Queen's Medical Centre, UK.

Country: NETHERLANDS

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MEDLINETA: FEMS Microbiol Lett

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