Molecular cloning and gene expression of chum salmon cystatin.

Molecular cloning and gene expression of chum salmon cystatin. Research Abstract Details 

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Molecular cloning and gene expression of chum salmon cystatin. Abstract Text:

M Yamashita,S Konagaya,

A salmon cystatin cDNA clone was isolated from a chum salmon cDNA library. The clone encoded a full-length extracellular-type cystatin and its signal peptide and included 5'- and 3'-untranslated regions. The deduced amino acid sequence showed a high degree of sequence similarity to mammalian cystatin C, chicken egg cystatin, and chum salmon pituitary cystatin. By Northern blot analysis, the salmon cystatin was found to show apparently non-tissue specific expression. Because platyfish EHS cells transfected with a cystatin expression vector produced a 13 kDa mature cystatin in the culture medium, the salmon cystatin was considered to act as an extracellular type of cystatin in the fish cells. These findings indicate that the salmon cystatin is a homolog of mammalian cystatin C.

Molecular cloning and gene expression of chum salmon cystatin. Publishing Authors By Initials

M Yamashita,S Konagaya,

For similar investigative techniques: genetic techniques: gene transfer techniques: transfection research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transfection research

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Molecular cloning and gene expression of chum salmon cystatin. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 120

Page Numbers: 483-7

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 1996

Molecular cloning and gene expression of chum salmon cystatin. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Molecular cloning and gene expression of chum salmon cystatin. Keywords Mesh Terms:

KEYWORDS: Transfection

MESH TERMS: chemistry

Chemical & Substance for Abstract: Molecular cloning and gene expression of chum salmon cystatin. Information

Substance Name: Recombinant Proteins

Registry Number: 0

Grant and Affiliation Information for Molecular cloning and gene expression of chum salmon cystatin.

AFFILIATION: National Research Institute of Fisheries Science, Yokohama. mic@nrifs.affrc.go.jp

Country: JAPAN

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MEDLINETA: J Biochem

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ACCESSION NUMBER: D86628

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