Molecular cloning and functional study of rat estrogen receptor-related receptor gamma in rat prostatic cells.

Molecular cloning and functional study of rat estrogen receptor-related receptor gamma in rat prostatic cells. Research Abstract Details 

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Molecular cloning and functional study of rat estrogen receptor-related receptor gamma in rat prostatic cells. Abstract Text:

Ki Lui,Yu Huang,H L Choi,Shan Yu,K B Wong,Shiuan Chen,Franky L Chan,

BACKGROUND: Based on high homology of ERRs with ERs, we hypothesize that ERRs might functionally cross talk with ERs or independently in prostatic cells. METHODS: We examined the ERRgamma expressions in rat prostates and Nb rat prostate cancer model, and its growth regulation in stable transfectants of prostatic cells. RESULTS: We cloned the ERRgamma cDNA from rat prostate by RACE-PCR. Its expression was confirmed by Northern and immunoblottings. Real-time RT-PCR showed that its expression in castrated prostates was androgen-dependent. ERRgamma was expressed in prostatic epithelial cells, but showed reduced expressions in neoplastic prostates. Transfections confirmed that ERRgamma was expressed in prostatic cells as nuclear protein and transcriptionally active without estradiol. Its overexpression in ERRgamma-stable transfectants of NbE-1 and MAT-Lu cells inhibited their in vitro proliferation, anchorage-independent growth in soft-agar and tumorigenicity in nude mice. CONCLUSIONS: Our studies show that ERRgamma is functionally expressed in rat prostate and may play anti-proliferative actions in prostatic cells. Its co-expression with ERs suggests that besides ERs, ligand-independent ERRgamma is also involved in prostatic growth and functions.

Molecular cloning and functional study of rat estrogen receptor-related receptor gamma in rat prostatic cells. Publishing Authors By Initials

K Lui,Y Huang,HL Choi,S Yu,KB Wong,S Chen,FL Chan,

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Molecular cloning and functional study of rat estrogen receptor-related receptor gamma in rat prostatic cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Prostate

VOLUME: 66

Page Numbers: 1600-19

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ISSN: 0270-4137

DAY: 1

MONTH: Nov

YEAR: 2006

Molecular cloning and functional study of rat estrogen receptor-related receptor gamma in rat prostatic cells. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Molecular cloning and functional study of rat estrogen receptor-related receptor gamma in rat prostatic cells.

AFFILIATION: Department of Anatomy, The Chinese University of Hong Kong, Hong Kong, China.

Country: United States

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MEDLINETA: Prostate

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