Molecular Cloning and Functional Analysis of the Gene Encoding 3-hydroxy-3-methylglutaryl Coenzyme A Reductase from Hazel (Corylus avellana L. Gasaway).

Molecular Cloning and Functional Analysis of the Gene Encoding 3-hydroxy-3-methylglutaryl Coenzyme A Reductase from Hazel (Corylus avellana L. Gasaway). Research Abstract Details 

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Molecular Cloning and Functional Analysis of the Gene Encoding 3-hydroxy-3-methylglutaryl Coenzyme A Reductase from Hazel (Corylus avellana L. Gasaway). Abstract Text:

Yechun Wang,Binhui Guo,Fei Zhang,Hongyan Yao,Zhiqi Miao,Kexuan Tang,Yechun Wang,Binhui Guo,Fei Zhang,Hongyan Yao,Zhiqi Miao,Kexuan Tang,Yechun Wang,Binhui Guo,Fei Zhang,Hongyan Yao,Zhiqi Miao,Kexuan Tang,

The enzyme 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR; EC1.1.1.34) catalyzes the first committed step of isoprenoids biosynthesis in MVA pathway. Here we report for the first time the cloning and characterization of a full-length cDNA encoding HMGR (designated as CgHMGR, GenBank accession number EF206343) from hazel (Corylus avellana L. Gasaway), a taxol-producing plant species. The full-length cDNA of CgHMGR was 2064 bp containing a 1704-bp ORF encoding 567 amino acids. Bioinformatic analyses revealed that the deduced CgHMGR had extensive homology with other plant HMGRs and contained two transmembrane domains and a catalytic domain. The predicted 3-D model of CgHMGR had a typical spatial structure of HMGRs. Southern blot analysis indicated that CgHMGR belonged to a small gene family. Expression analysis revealed that CgHMGR expressed high in roots, and low in leaves and stems, and the expression of CgHMGR could be up-regulated by methyl jasmonate (MeJA). The functional color assay in Escherichia coli showed that CgHMGR could accelerate the biosynthesis of beta-carotene, indicating that CgHMGR encoded a functional protein. The cloning, characterization and functional analysis of CgHMGR gene will enable us to further understand the role of CgHMGR involved in taxol biosynthetic pathway in C. avellana at molecular level.

Molecular Cloning and Functional Analysis of the Gene Encoding 3-hydroxy-3-methylglutaryl Coenzyme A Reductase from Hazel (Corylus avellana L. Gasaway). Publishing Authors By Initials

Y Wang,B Guo,F Zhang,H Yao,Z Miao,K Tang,Y Wang,B Guo,F Zhang,H Yao,Z Miao,K Tang,Y Wang,B Guo,F Zhang,H Yao,Z Miao,K Tang,

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Molecular Cloning and Functional Analysis of the Gene Encoding 3-hydroxy-3-methylglutaryl Coenzyme A Reductase from Hazel (Corylus avellana L. Gasaway). Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry and molecular biology

VOLUME: 40

Page Numbers: 861-9

Journal Abbreviation: J. Biochem. Mol. Biol.

ISSN: 1225-8687

DAY: 30

MONTH: Nov

YEAR: 2007

Molecular Cloning and Functional Analysis of the Gene Encoding 3-hydroxy-3-methylglutaryl Coenzyme A Reductase from Hazel (Corylus avellana L. Gasaway). Information

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LANGUAGE: eng

NlmUniqueID: 9702084

Molecular Cloning and Functional Analysis of the Gene Encoding 3-hydroxy-3-methylglutaryl Coenzyme A Reductase from Hazel (Corylus avellana L. Gasaway). Keywords Mesh Terms:

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Grant and Affiliation Information for Molecular Cloning and Functional Analysis of the Gene Encoding 3-hydroxy-3-methylglutaryl Coenzyme A Reductase from Hazel (Corylus avellana L. Gasaway).

AFFILIATION: Plant Biotechnology Research Center, School of Agriculture and Biology, School of Life Science and Technology, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Shanghai Jiao Tong University, Shanghai 200030, PR China kxtang1@yahoo.com.

Country: Korea (South)

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MEDLINETA: J Biochem Mol Biol

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