Molecular cloning and characterization of Crmdr1, a novel MDR-type ABC transporter gene from Catharanthus roseus.

Molecular cloning and characterization of Crmdr1, a novel MDR-type ABC transporter gene from Catharanthus roseus. Research Abstract Details 

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Molecular cloning and characterization of Crmdr1, a novel MDR-type ABC transporter gene from Catharanthus roseus. Abstract Text:

Hongbin Jin,Donghui Liu,Kaijing Zuo,Yifu Gong,Zhiqi Miao,Yuhui Chen,Weiwei Ren,Xiaofen Sun,Kexuan Tang,Hongbin Jin,Donghui Liu,Kaijing Zuo,Yifu Gong,Zhiqi Miao,Yuhui Chen,Weiwei Ren,Xiaofen Sun,Kexuan Tang,

A novel gene encoding a MDR-like ABC transporter protein was cloned from Catharanthus roseus, a medicinal plant with more than 120 kinds of secondary metabolites, through rapid amplification of cDNA ends (RACE). This gene (named as Crmdr1; GenBank accession no.: DQ660356) had a total length of 4395 bp with an open reading frame of 3801 bp, and encoded a predicted polypeptide of 1266 amino acids with a molecular weight of 137.1 kDa. The CrMDR1 protein shared 59.8, 62.5, 60.0 and 58.2% identity with other MDR proteins isolated from Arabidopsis thaliana (AAD31576), Coptis japonica (CjMDR), Gossypium hirsutum (GhMDR) and Triticum aestivum (TaMDR) at amino acid level, respectively. Southern blot analysis showed that Crmdr1 was a low-copy gene. Expression pattern analysis revealed that Crmdr1 constitutively expressed in the root, stem and leaf, but with lower expression in leaf. The domains analysis showed that CrMDR1 protein possessed two transmembrane domains (TMDs) and two nucleotide binding domains (NBDs) arranging in "TMD1-NBD1-TMD2-NBD2" direction, which is consistent with other MDR transporters. Within NBDs three characteristic motifs common to all ABC transporters, "Walker A", "Walker B" and C motif, were found. These results indicate that CrMDR1 is a MDR-like ABC transporter protein that may be involved in the transport and accumulation of secondary metabolites.

Molecular cloning and characterization of Crmdr1, a novel MDR-type ABC transporter gene from Catharanthus roseus. Publishing Authors By Initials

H Jin,D Liu,K Zuo,Y Gong,Z Miao,Y Chen,W Ren,X Sun,K Tang,H Jin,D Liu,K Zuo,Y Gong,Z Miao,Y Chen,W Ren,X Sun,K Tang,

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Molecular cloning and characterization of Crmdr1, a novel MDR-type ABC transporter gene from Catharanthus roseus. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: DNA sequence : the journal of DNA sequencing and m

VOLUME: 18

Page Numbers: 316-25

Journal Abbreviation: DNA Seq.

ISSN: 1042-5179

DAY: 1

MONTH: Aug

YEAR: 2007

Molecular cloning and characterization of Crmdr1, a novel MDR-type ABC transporter gene from Catharanthus roseus. Information

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LANGUAGE: eng

NlmUniqueID: 9107800

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Grant and Affiliation Information for Molecular cloning and characterization of Crmdr1, a novel MDR-type ABC transporter gene from Catharanthus roseus.

AFFILIATION: School of Agriculture and Biology, Shangha Jiao Tong University, Shanghai, People's Republic of China.

Country: England

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MEDLINETA: DNA Seq

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