Molecular cloning and characterization of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (CaHDR) from Camptotheca acuminata and its functional identification in Escherichia coli.

Molecular cloning and characterization of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (CaHDR) from Camptotheca acuminata and its functional identification in Escherichia coli. Research Abstract Details 

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Molecular cloning and characterization of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (CaHDR) from Camptotheca acuminata and its functional identification in Escherichia coli. Abstract Text:

Qian Wang,Yan Pi,Rong Hou,Keji Jiang,Zhuoshi Huang,Ming-Shiun Hsieh,Xiaofen Sun,Kexuan Tang,

Camptothecin is an anti-cancer monoterpene indole alkaloid. The gene encoding 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (designated as CaHDR), the last catalytic enzyme of the MEP pathway for terpenoid biosynthesis, was isolated from camptothecin-producing Camptotheca acuminata. The full-length cDNA of CaHDR was 1686 bp encoding 459 amino acids. Comparison of the cDNA and genomic DNA of CaHDR revealed that there was no intron in genomic CaHDR. Southern blot analysis indicated that CaHDR belonged to a low-copy gene family. RT-PCR analysis revealed that CaHDR expressed constitutively in all tested plant organs with the highest expression level in flowers, and the expression of CaHDR could be induced by 100 muM methyl-jasmonate (MeJA), but not by 100 mg/L salicylic acid (SA) in the callus of C. acuminata. The complementation of CaHDR in Escherichia coli ispH mutant MG1655 demonstrated its function. [BMB reports 2008; 41(2): 112-118].

Molecular cloning and characterization of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (CaHDR) from Camptotheca acuminata and its functional identification in Escherichia coli. Publishing Authors By Initials

Q Wang,Y Pi,R Hou,K Jiang,Z Huang,MS Hsieh,X Sun,K Tang,

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Molecular cloning and characterization of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (CaHDR) from Camptotheca acuminata and its functional identification in Escherichia coli. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: BMB reports

VOLUME: 41

Page Numbers: 112-8

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ISSN: 1976-6696

DAY: 4

MONTH: Feb

YEAR: 2008

Molecular cloning and characterization of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (CaHDR) from Camptotheca acuminata and its functional identification in Escherichia coli. Information

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LANGUAGE: eng

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Molecular cloning and characterization of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (CaHDR) from Camptotheca acuminata and its functional identification in Escherichia coli. Keywords Mesh Terms:

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Grant and Affiliation Information for Molecular cloning and characterization of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (CaHDR) from Camptotheca acuminata and its functional identification in Escherichia coli.

AFFILIATION: State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center,Morgan-Tan International Center for Life Sciences, Fudan University, Shanghai, People's Republic of China kxtang1@yahoo.com.

Country: Korea (South)

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Molecular cloning and characterization of 1-hydroxy-2-methyl-2-E-butenyl 4-diphosphate reductase CaHDR from Camptotheca acuminata and its functional identification in Escherichia coli Related Publications

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