Molecular characterization of woodchuck type I interferons and their expression by woodchuck peripheral blood lymphocytes.

Molecular characterization of woodchuck type I interferons and their expression by woodchuck peripheral blood lymphocytes. Research Abstract Details 

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Molecular characterization of woodchuck type I interferons and their expression by woodchuck peripheral blood lymphocytes. Abstract Text:

Yinping Lu,Yang Xu,Dongliang Yang,Thekla Kemper,Michael Roggendorf,Mengji Lu,Yinping Lu,Yang Xu,Dongliang Yang,Thekla Kemper,Michael Roggendorf,Mengji Lu,

Interferon (IFN)-alpha and -beta are important antiviral mediators. IFN-alpha is widely used for the treatment of chronic hepatitis B. In our previous studies, a subtype of woodchuck IFN-alpha (wIFN-alpha) was characterized and has been shown to be active in suppressing the replication of woodchuck hepatitis virus (WHV) in vitro and vivo. Here, we refined the analysis of the IFN-alpha/beta system of the woodchuck and studied the expression of wIFN-alpha/beta in peripheral blood lymphocytes (PBLs) from naïve and WHV-infected woodchucks. A number of wIFN-alpha genes were sequenced and could be classified into 10 subtypes and 3 pseudotypes. The biological activity of different subtypes of wIFN-alpha was demonstrated by their ability to protect woodchuck cells against encephalomyocarditis virus infection and to induce MxA expression in woodchuck cells. Additionally, a partial sequence of wIFN-beta was characterized. A subtyping method for wIFN-alpha based on restriction length polymorphism analysis was developed. Further, the expression of wIFN by woodchuck PBLs after stimulation with polyI/C was investigated. The maximal production of wIFN by woodchuck PBLs occurred within the first 48 h after addition poly I/C. The wIFN-alpha subtypes 1, 4, and 5 were found to be produced by poly I/C-stimulated woodchuck PBLs, indicating a selective expression of wIFN-alpha subtypes. PBLs from chronically WHV-infected woodchucks showed a reduced ability to produce wIFN when stimulated with poly I/C. The results suggest that woodchucks with chronic WHV infection have impaired immunological responses to poly I/C.

Molecular characterization of woodchuck type I interferons and their expression by woodchuck peripheral blood lymphocytes. Publishing Authors By Initials

Y Lu,Y Xu,D Yang,T Kemper,M Roggendorf,M Lu,Y Lu,Y Xu,D Yang,T Kemper,M Roggendorf,M Lu,

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Molecular characterization of woodchuck type I interferons and their expression by woodchuck peripheral blood lymphocytes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Cytokine

VOLUME: 41

Page Numbers: 127-35

Journal Abbreviation: Cytokine

ISSN: 1096-0023

DAY: 21

MONTH: 12

YEAR: 2007

Molecular characterization of woodchuck type I interferons and their expression by woodchuck peripheral blood lymphocytes. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Molecular characterization of woodchuck type I interferons and their expression by woodchuck peripheral blood lymphocytes.

AFFILIATION: Institut für Virologie, Universitätsklinikum Essen, Hufeland str. 55, 45122 Essen, Germany.

Country: United States

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MEDLINETA: Cytokine

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