Molecular characterization of trichomonads from feces of dogs with diarrhea.

Molecular characterization of trichomonads from feces of dogs with diarrhea. Research Abstract Details 

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Molecular characterization of trichomonads from feces of dogs with diarrhea. Abstract Text:

Jody L Gookin,Adam J Birkenheuer,Victoria St John,Michelle Spector,Michael G Levy,

Trichomonads are occasionally observed in the feces of dogs with diarrhea. On the basis of superficial morphological appearance, these infections have been attributed to opportunistic overgrowth of the commensal, Pentatrichomonas hominis. However, molecular characterization of canine trichomonads has never been reported. This study was performed to determine, by means of rRNA gene sequence analysis, the identity of trichomonads observed in feces from dogs with diarrhea. Total DNA was isolated from fecal samples obtained from a 3-mo-old mixed breed dog and litter of German Shepherd puppies having profuse liquid diarrhea containing numerous trichomonads. Total DNA was subject to PCR amplification of partial 18S rRNA gene or 5.8S, ITS1, ITS2, and partial 18S and 28S rRNA genes using species-specific and universal primers, respectively. Products of 642 and 1864 base-pair length were amplified and cloned. On the basis of rRNA gene sequence, the trichomonads observed in the single dog and the litter of puppies shared 100% identity with Tritrichomonas foetus and P. hominis, respectively. The present study is the first to establish the molecular identity of trichomonads infecting dogs with diarrhea. These studies validate the longstanding assumption that canine trichomoniasis may be attributed to P. hominis. Importantly, these studies additionally recognize that canine trichomoniasis may also be caused by infection with T. foetus.

Molecular characterization of trichomonads from feces of dogs with diarrhea. Publishing Authors By Initials

JL Gookin,AJ Birkenheuer,V St John,M Spector,MG Levy,

For similar animals: invertebrates: protozoa: sarcomastigophora: mastigophora: zoomastigophora: trichomonadida: tritrichomonas: tritrichomonas foetus research abstracts see: animals: invertebrates: protozoa: sarcomastigophora: mastigophora: zoomastigophora: trichomonadida: tritrichomonas: tritrichomonas foetus research

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Molecular characterization of trichomonads from feces of dogs with diarrhea. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of parasitology

VOLUME: 91

Page Numbers: 939-43

Journal Abbreviation: J. Parasitol.

ISSN: 0022-3395

DAY: 3

MONTH: Aug

YEAR: 2005

Molecular characterization of trichomonads from feces of dogs with diarrhea. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7803124

Molecular characterization of trichomonads from feces of dogs with diarrhea. Keywords Mesh Terms:

KEYWORDS: Tritrichomonas foetus

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Molecular characterization of trichomonads from feces of dogs with diarrhea. Information

Substance Name: RNA, Ribosomal

Registry Number: 0

Grant and Affiliation Information for Molecular characterization of trichomonads from feces of dogs with diarrhea.

AFFILIATION: Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606, USA. jody_gookin@ncsu.edu

Country: United States

AGENCY: United States NIDDK

GRANT: DK02868

ACRONYM: DK

MEDLINETA: J Parasitol

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