Molecular characterization of duck hepatitis B virus isolated from Hubei brown ducks.

Molecular characterization of duck hepatitis B virus isolated from Hubei brown ducks. Research Abstract Details 

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Molecular characterization of duck hepatitis B virus isolated from Hubei brown ducks. Abstract Text:

Quan Hu,Xiaoyong Zhang,Yangchang Lei,Zhengmao Zhang,Lu Mengji,Dongliang Yang,

The objective of this study was to characterize the genome structure of duck hepatitis B virus (DHBV) isolated from Hubei brown ducks. The natural carrier rate of DHBV in adult ducks from Hubei area was investigated and the DHBV DNA-positive serum screened out. The complete genome of a DHBV strain was amplified by polymerase chain reaction (PCR) and cloned into T vector and sequenced. The results showed that the carrier rate of DHBV in Hubei brown ducks was 10 %. This strain (GenBank accession number DQ276978) had a genome of 3024 nucleotides with three overlapping open reading frames encoding the surface, core and polymerase proteins respectively. Comparison of the strain with 17 DHBV strains registered in GenBank revealed a homology from 89.3 % to 93.5 % at the nucleotide level. The sequences of the structural and functional domains of these proteins were highly conserved. The strain was found to share more signature amino acids in the polymerase genes with the "Chinese" DHBV strains than those of the "Western" country strains. This finding was also corroborated by a phylogenetic tree analysis. Therefore, the DQ276978 might belong to a subtype of the Chinese DHBV strains.

Molecular characterization of duck hepatitis B virus isolated from Hubei brown ducks. Publishing Authors By Initials

Q Hu,X Zhang,Y Lei,Z Zhang,L Mengji,D Yang,

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Molecular characterization of duck hepatitis B virus isolated from Hubei brown ducks. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of Huazhong University of Science and Tech

VOLUME: 26

Page Numbers: 633-6

Journal Abbreviation: J. Huazhong Univ. Sci. Technol

ISSN: 1672-0733

DAY: 15

MONTH: 01

YEAR: 2006

Molecular characterization of duck hepatitis B virus isolated from Hubei brown ducks. Information

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LANGUAGE: eng

NlmUniqueID: 101169627

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Grant and Affiliation Information for Molecular characterization of duck hepatitis B virus isolated from Hubei brown ducks.

AFFILIATION: Division of Clinical Immunology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

Country: China

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MEDLINETA: J Huazhong Univ Sci Technolog

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ACCESSION NUMBER: DQ276978

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