Molecular characterization and nuclear localization of rat timeless-like gene product.

Molecular characterization and nuclear localization of rat timeless-like gene product. Research Abstract Details 

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Molecular characterization and nuclear localization of rat timeless-like gene product. Abstract Text:

S Sakamoto,K Miyazaki,H Fukui,K Oishi,N Hayasaka,M Okada,M Kamakura,T Taniguchi,K Nagai,N Ishida,

Among three period genes (per1, per2, per3) in mammals, only per2 gene was shown to be involved in the core clock mechanism. To elucidate the molecular function of rat PERIOD2 (rPER2), we searched for binding proteins to the PAS domain of rPER2. We isolated a binding protein to this domain and identified it as a TIMELESS-like protein (TLP) on the basis of mass analyses. Then, we isolated a rat TLP cDNA from the rat hypothalamus library. RNA blot analysis and in situ hybridization indicates that rTLP mRNA was expressed in all rat tissues from whole brain, the suprachiasmatic nucleus, eye, lung, heart, liver, kidney, placenta, and testis. When rTLP gene product was expressed in COS-1 cells, nuclear localization of rTLP was detected in 99.6% of transfected cells. These results suggest that the interaction of rPER2 with rTLP may influence the regulation of circadian clock components in nucleus after rPER2 is translocated into the nucleus.

Molecular characterization and nuclear localization of rat timeless-like gene product. Publishing Authors By Initials

S Sakamoto,K Miyazaki,H Fukui,K Oishi,N Hayasaka,M Okada,M Kamakura,T Taniguchi,K Nagai,N Ishida,

For similar proteins: transcription factors research abstracts see: proteins: transcription factors research

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Molecular characterization and nuclear localization of rat timeless-like gene product. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochemical and biophysical research communication

VOLUME: 279

Page Numbers: 131-8

Journal Abbreviation: Biochem. Biophys. Res. Commun.

ISSN: 0006-291X

DAY: 9

MONTH: Dec

YEAR: 2000

Molecular characterization and nuclear localization of rat timeless-like gene product. Information

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LANGUAGE: eng

NlmUniqueID: 372516

Molecular characterization and nuclear localization of rat timeless-like gene product. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: Molecular characterization and nuclear localization of rat timeless-like gene product. Information

Substance Name: timeless protein, Drosophila

Registry Number: 0

Grant and Affiliation Information for Molecular characterization and nuclear localization of rat timeless-like gene product.

AFFILIATION: Laboratory of Molecular Biology, Medical Research Center, Kochi Medical School, Okoh, Nankoku, Kochi 783-8505, Japan.

Country: UNITED STATES

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MEDLINETA: Biochem Biophys Res Commun

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