Molecular characterization and detection of plum bark necrosis stem pitting-associated virus.

Molecular characterization and detection of plum bark necrosis stem pitting-associated virus. Research Abstract Details 

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Molecular characterization and detection of plum bark necrosis stem pitting-associated virus. Abstract Text:

M Al Rwahnih,J K Uyemoto,B W Falk,A Rowhani,M Al Rwahnih,J K Uyemoto,B W Falk,A Rowhani,

The complete RNA genome of plum bark necrosis stem pitting-associated virus (PBNSPaV) was cloned and sequenced and was determined to be 14, 214 nts long. The genome structure revealed seven major open reading frames (ORFs), and nontranslated regions at the 5' and 3' ends. PBNSPaV represents the simplest genome organization in the genus Ampelovirus, family Closteroviridae. The ORFs 1a and 1b encode, respectively, a large polyprotein with a molecular mass (Mr) of 259.6 kDa containing conserved domains characteristic of a papain-like protease, methyltransferase and helicase (ORF1a) and a 64.1-kDa protein of eight conserved motifs characteristic of viral RNA-dependent RNA polymerase (RdRp) (ORF1b). ORF1b is presumably expressed via a +1 ribosomal frameshift mechanism. ORF2 encodes a small 6.3-kDa hydrophobic protein of unknown function. ORF3 encodes a 57.4-kDa protein, a homologue of the HSP70 family of heat shock proteins. ORF4 encodes a 61.6-kDa protein with unknown function. ORF5 encodes a 35.9-kDa capsid protein (CP). Lastly, ORF6 encodes a 25.2-kDa minor capsid protein (CPm). Phylogenetic analyses performed on sequences of the HSP70h RdRp and CP support classification of the virus in the genus Ampelovirus. A real-time TaqMan RT-PCR assay and a one-step RT-PCR were developed for PBNSPaV detection and compared using three different sample preparation methods.

Molecular characterization and detection of plum bark necrosis stem pitting-associated virus. Publishing Authors By Initials

M Al Rwahnih,JK Uyemoto,BW Falk,A Rowhani,M Al Rwahnih,JK Uyemoto,BW Falk,A Rowhani,

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

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Molecular characterization and detection of plum bark necrosis stem pitting-associated virus. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Archives of virology

VOLUME: 152

Page Numbers: 2197-206

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ISSN: 0304-8608

DAY: 21

MONTH: 09

YEAR: 2007

Molecular characterization and detection of plum bark necrosis stem pitting-associated virus. Information

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LANGUAGE: eng

NlmUniqueID: 7506870

Molecular characterization and detection of plum bark necrosis stem pitting-associated virus. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: genetics

Chemical & Substance for Abstract: Molecular characterization and detection of plum bark necrosis stem pitting-associated virus. Information

Substance Name: Taq Polymerase

Registry Number: EC 2.7.7.-

Grant and Affiliation Information for Molecular characterization and detection of plum bark necrosis stem pitting-associated virus.

AFFILIATION: Department of Plant Pathology, University of California, Davis, CA 95616, USA.

Country: Austria

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MEDLINETA: Arch Virol

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