Molecular basis for control of conjugation by bacterial pheromone and inhibitor peptides.

Molecular basis for control of conjugation by bacterial pheromone and inhibitor peptides. Research Abstract Details 

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Molecular basis for control of conjugation by bacterial pheromone and inhibitor peptides. Abstract Text:

Briana K Kozlowicz,Ke Shi,Zu-Yi Gu,Douglas H Ohlendorf,Cathleen A Earhart,Gary M Dunny,

In many bacteria expression of lateral gene transfer and of virulence factors is controlled by cell-cell signalling systems. Molecular interactions of microbial signal molecules with their cognate receptors are not well understood. For the Enterococcus faecalis conjugative plasmid pCF10, the PrgX protein serves as a molecular switch controlling expression of conjugation and virulence genes encoded by the plasmid. The induction state of a pCF10-carrying donor cell is determined by the ratio of two signalling peptides, cCF10 pheromone and iCF10 inhibitor. Recent analysis of PrgX/cCF10 interactions suggests a mechanism for conversion to the induced state. However, the means by which iCF10 peptide antagonizes cCF10 activity is unclear, and it has been suggested that inhibitor peptides block import of pheromone peptides. We now show that both of these peptides interact with the same binding pocket of PrgX, but they differentially alter the conformation of the protein and its oligomerization state, resulting in opposing biological activities.

Molecular basis for control of conjugation by bacterial pheromone and inhibitor peptides. Publishing Authors By Initials

BK Kozlowicz,K Shi,ZY Gu,DH Ohlendorf,CA Earhart,GM Dunny,

For similar proteins: membrane proteins: receptors, cell surface: receptors, peptide research abstracts see: proteins: membrane proteins: receptors, cell surface: receptors, peptide research

PUBMED ID PMID:

MEDLINE DATE:

Molecular basis for control of conjugation by bacterial pheromone and inhibitor peptides. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular microbiology

VOLUME: 62

Page Numbers: 958-69

Journal Abbreviation: Mol. Microbiol.

ISSN: 0950-382X

DAY: 13

MONTH: 10

YEAR: 2006

Molecular basis for control of conjugation by bacterial pheromone and inhibitor peptides. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8712028

Molecular basis for control of conjugation by bacterial pheromone and inhibitor peptides. Keywords Mesh Terms:

KEYWORDS: Receptors, Peptide

MESH TERMS: physiology

Chemical & Substance for Abstract: Molecular basis for control of conjugation by bacterial pheromone and inhibitor peptides. Information

Substance Name: peptide pheromone cCF10

Registry Number: 0

Grant and Affiliation Information for Molecular basis for control of conjugation by bacterial pheromone and inhibitor peptides.

AFFILIATION: Department of Microbiology, 1460 Mayo Memorial Building, University of Minnesota, Minneapolis, MN 55455, USA.

Country: England

AGENCY: United States NIGMS

GRANT: T32 GM08347

ACRONYM: GM

MEDLINETA: Mol Microbiol

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Molecular basis for control of conjugation by bacterial pheromone and inhibitor peptides Related Publications

• Analysis of the amino acid sequence specificity determinants of the enterococcal cCF10 sex pheromone in interactions with the pheromone-sensing machinery.
• Characterization of the sequence specificity determinants required for processing and control of sex pheromone by the intramembrane protease Eep and the plasmid-encoded protein PrgY.
• Development and use of an efficient system for random mariner transposon mutagenesis to identify novel genetic determinants of biofilm formation in the core Enterococcus faecalis genome.
• Development of a host-genotype-independent counterselectable marker and a high-frequency conjugative delivery system and their use in genetic analysis of Enterococcus faecalis.
• Molecular basis for control of conjugation by bacterial pheromone and inhibitor peptides.
• Specificity determinants of conjugative DNA processing in the Enterococcus faecalis plasmid pCF10 and the Lactococcus lactis plasmid pRS01.
• The peptide pheromone-inducible conjugation system of Enterococcus faecalis plasmid pCF10: cell-cell signalling, gene transfer, complexity and evolution.