Molecular architecture of botulinum neurotoxin e revealed by single particle electron microscopy.

Molecular architecture of botulinum neurotoxin e revealed by single particle electron microscopy. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Molecular architecture of botulinum neurotoxin e revealed by single particle electron microscopy. Abstract Text:

Audrey Fischer,Consuelo Garcia-Rodriguez,Isin Geren,Jianlong Lou,James D Marks,Terunaga Nakagawa,Mauricio Montal,

Clostridial botulinum neurotoxin (BoNT) causes a neuroparalytic condition recognized as botulism by arresting synaptic vesicle exocytosis. Although the crystal structures of full-length BoNT/A and BoNT/B holotoxins are known, the molecular architecture of the five other serotypes remains elusive. Here, we present the structures of BoNT/A and BoNT/E using single particle electron microscopy. Labeling of the particles with three different monoclonal antibodies raised against BoNT/E revealed the positions of their epitopes in the electron microscopy structure, thereby identifying the three hallmark domains of BoNT (protease, translocation, and receptor binding). Correspondingly, these antibodies selectively inhibit BoNT translocation activity as detected using a single molecule assay. The global structure of BoNT/E is strikingly different from that of BoNT/A despite strong sequence similarity. We postulate that the unique architecture of functionally conserved modules underlies the distinguishing attributes of BoNT/E and contributes to differences with BoNT/A.

Molecular architecture of botulinum neurotoxin e revealed by single particle electron microscopy. Publishing Authors By Initials

A Fischer,C Garcia-Rodriguez,I Geren,J Lou,JD Marks,T Nakagawa,M Montal,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Molecular architecture of botulinum neurotoxin e revealed by single particle electron microscopy. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of biological chemistry

VOLUME: 283

Page Numbers: 3997-4003

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 20

MONTH: 11

YEAR: 2007

Molecular architecture of botulinum neurotoxin e revealed by single particle electron microscopy. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Molecular architecture of botulinum neurotoxin e revealed by single particle electron microscopy. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Molecular architecture of botulinum neurotoxin e revealed by single particle electron microscopy. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Molecular architecture of botulinum neurotoxin e revealed by single particle electron microscopy.

AFFILIATION: Section of Neurobiology, Division of Biological Sciences.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Molecular architecture of botulinum neurotoxin e revealed by single particle electron microscopy Related Publications

• Crucial role of the disulfide bridge between botulinum neurotoxin light and heavy chains in protease translocation across membranes.
• Molecular architecture of botulinum neurotoxin e revealed by single particle electron microscopy.
• Molecular template for a voltage sensor in a novel K+ channel. I. Identification and functional characterization of KvLm, a voltage-gated K+ channel from Listeria monocytogenes.
• Molecular template for a voltage sensor in a novel K+ channel. II. Conservation of a eukaryotic sensor fold in a prokaryotic K+ channel.
• Single molecule detection of intermediates during botulinum neurotoxin translocation across membranes.