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Molecular and morphological discrimination of stipitate hydnoids in the genera Hydnellum and Phellodon.

Molecular and morphological discrimination of stipitate hydnoids in the genera Hydnellum and Phellodon. Research Abstract Details 

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  • Molecular and morphological discrimination of stipitate hydnoids in the genera Hydnellum and Phellodon. Abstract Text:

    david parfittDavid Parfitt,a martyn ainsworthA Martyn Ainsworth,deborah simpsonDeborah Simpson,hilary j rogersHilary J Rogers,lynne boddyLynne Boddy,

    Hydnellum and Phellodon species, generally considered ectomycorrhizal partners of a range of woody angiosperms and gymnosperms particularly within Fagaceae and Pinaceae, appear to be declining in continental Europe. They are listed as priority species in the UK Biodiversity Action Plan, but their UK conservation status remains uncertain. Interpretation of species distribution data is hampered by a lack of consensus regarding some key discriminatory morphological characters and difficulties with their interpretation. DNA sequencing of the ITS1 region of the ribosomal gene cluster discriminated between the known British species of Phellodon but revealed more terminal clusters than currently recognised taxa. Although the main focus within Hydnellum was on the very similar species pair H. concrescens and H. scrobiculatum, a few samples of H. caeruleum, H. ferrugineum, H. peckii, and H. spongiosipes were included in the study for reference. DNA sequencing of material identified on spore-based criteria as H. concrescens yielded two main groups, but samples received as H. scrobiculatum were generally more variable. Of these, two were reassigned and the remaining group, with very similar spores (although shorter than in published descriptions of H. scrobiculatum), had highly variable sequence data. The results and conservation importance of these fungi highlight the need for a taxonomic reassessment of P. melaleucus, P. niger, H. concrescens, and H. scrobiculatum collections from Britain and continental Europe using a combined molecular and morphological approach. Specific PCR primers were constructed to discriminate fruit bodies, mycelium, and mycorrhizal roots of P. niger and P. confluens from each other and from other stipitate hydnoids.

    Molecular and morphological discrimination of stipitate hydnoids in the genera Hydnellum and Phellodon. Publishing Authors By Initials

    d parfittD Parfitt,a martyn ainsworthA Martyn Ainsworth,d simpsonD Simpson,hj rogersHJ Rogers,l boddyL Boddy,

    For similar cells: spores: spores, fungal research abstracts see: cells: spores: spores, fungal research

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    Molecular and morphological discrimination of stipitate hydnoids in the genera Hydnellum and Phellodon. Journal Published:

    PUBLICATION TYPE: Research Support, Non-U.S. Gov

    Journal: Mycological research

    VOLUME: 111

    Page Numbers: 761-77

    Journal Abbreviation: Mycol. Res.

    ISSN: 0953-7562

    DAY: 18

    MONTH: 05

    YEAR: 2007

    Molecular and morphological discrimination of stipitate hydnoids in the genera Hydnellum and Phellodon. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 8913481

    Molecular and morphological discrimination of stipitate hydnoids in the genera Hydnellum and Phellodon. Keywords Mesh Terms:

    KEYWORDS: Spores, Fungal

    MESH TERMS: ultrastructure

    Chemical & Substance for Abstract: Molecular and morphological discrimination of stipitate hydnoids in the genera Hydnellum and Phellodon. Information

    Substance Name: DNA, Ribosomal Spacer

    Registry Number: 0

    Grant and Affiliation Information for Molecular and morphological discrimination of stipitate hydnoids in the genera Hydnellum and Phellodon.

    AFFILIATION: Cardiff School of Biosciences, Cardiff University, Cardiff CF10 3TL, UK.

    Country: England

    England Research PublicationEngland Research Publication

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    MEDLINETA: Mycol Res

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