Molecular and cellular characterization of ABCG2 in the prostate.

Molecular and cellular characterization of ABCG2 in the prostate. Research Abstract Details 

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Molecular and cellular characterization of ABCG2 in the prostate. Abstract Text:

Laura E Pascal,Asa J Oudes,Timothy W Petersen,Young Ah Goo,Laura S Walashek,Lawrence D TRUE,Alvin Y Liu,

BACKGROUND: Identification and characterization of the prostate stem cell is important for understanding normal prostate development and carcinogenesis. The flow cytometry-based side population (SP) technique has been developed to isolate putative adult stem cells in several human tissue types including the prostate. This phenotype is mainly mediated by the ATP-binding cassette membrane transporter ABCG2. METHODS: Immunolocalization of ABCG2 was performed on normal prostate tissue obtained from radical prostatectomies. Normal human prostate SP cells and ABCG2+ cells were isolated and gene expression was determined with DNA array analysis and RT-PCR. Endothelial cells were removed by pre-sorting with CD31. RESULTS: ABCG2 positive cells were localized to the prostate basal epithelium and endothelium. ABCG2+ cells in the basal epithelium constituted less than 1% of the total basal cell population. SP cells constituted 0.5-3% of the total epithelial fraction. The SP transcriptome was essentially the same as ABCG2+ and both populations expressed genes indicative of a stem cell phenotype, however, the cells also expressed many genes in common with endothelial cells. CONCLUSION: These results provide gene expression profiles for the prostate SP and ABCG2+ cells that will be critical for studying normal development and carcinogenesis, in particular as related to the cancer stem cell concept.

Molecular and cellular characterization of ABCG2 in the prostate. Publishing Authors By Initials

LE Pascal,AJ Oudes,TW Petersen,YA Goo,LS Walashek,LD TRUE,AY Liu,

For similar cells: stem cells research abstracts see: cells: stem cells research

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Molecular and cellular characterization of ABCG2 in the prostate. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: BMC urology

VOLUME: 7

Page Numbers: 6

Journal Abbreviation:

ISSN: 1471-2490

DAY: 10

MONTH: 04

YEAR: 2007

Molecular and cellular characterization of ABCG2 in the prostate. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100968571

Molecular and cellular characterization of ABCG2 in the prostate. Keywords Mesh Terms:

KEYWORDS: Stem Cells

MESH TERMS: cytology

Chemical & Substance for Abstract: Molecular and cellular characterization of ABCG2 in the prostate. Information

Substance Name: Neoplasm Proteins

Registry Number: 0

Grant and Affiliation Information for Molecular and cellular characterization of ABCG2 in the prostate.

AFFILIATION: Department of Urology, and the Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA 98195, USA. pascal2@u.washington.edu

Country: England

AGENCY: United States NIDDK

GRANT: DK63630

ACRONYM: DK

MEDLINETA: BMC Urol

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