Modulation of murine gastric antrum smooth muscle STOC activity and excitability by phospholamban.

Modulation of murine gastric antrum smooth muscle STOC activity and excitability by phospholamban. Research Abstract Details 

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Modulation of murine gastric antrum smooth muscle STOC activity and excitability by phospholamban. Abstract Text:

We investigated intracellular Ca(2+) waves, spontaneous transient outward currents (STOCs), and membrane potentials of gastric antrum smooth muscle cells from wild-type and phospholamban-knockout mice. The NO donor sodium nitroprusside (SNP) increased intracellular Ca(2+) wave activity in wild-type antrum smooth muscle cells, but had no effect on the constitutively elevated intracellular Ca(2+) wave activity of phospholamban-knockout cells. STOC activity was also constitutively elevated in phospholamban-knockout antrum smooth muscle cells relative to wild-type cells. SNP or 8-bromo-cGMP increased the STOC activity of wild-type antrum smooth muscle cells, but had no effect on STOC activity of phospholamban-knockout cells. Iberiotoxin, but not apamin, inhibited STOC activity in wild-type and phospholamban-knockout antrum smooth muscle cells. In the presence of SNP, STOC activity in wild-type and phospholamban-knockout antrum smooth muscle cells was inhibited by ryanodine, but not 2-APB. The cGMP-dependent protein kinase inhibitor KT5823 reversed the increase in STOC activity evoked by SNP in wild-type antrum smooth muscle cells, but had no effect on STOC activity in phospholamban-knockout cells. The resting membrane potential of phospholamban-knockout antrum smooth muscle cells was hyperpolarized by approximately -6 mV compared to wild-type cells. SNP hyperpolarized the resting membrane potential of wild-type antrum smooth muscle cells to a greater extent than phospholamban-knockout antrum smooth muscles. Despite the hyperpolarized membrane potential, slow wave activity was significantly increased in phospholamban-knockout antrum smooth muscles compared to wild-type smooth muscles. These results suggest that phospholamban is an important component of the mechanisms regulating the electrical properties of gastric antrum smooth muscles.

Modulation of murine gastric antrum smooth muscle STOC activity and excitability by phospholamban. Publishing Authors By Initials

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Modulation of murine gastric antrum smooth muscle STOC activity and excitability by phospholamban. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of physiology

VOLUME: 586

Page Numbers: 4977-91

Journal Abbreviation: J. Physiol. (Lond.)

ISSN: 1469-7793

DAY: 28

MONTH: 08

YEAR: 2008

Modulation of murine gastric antrum smooth muscle STOC activity and excitability by phospholamban. Information

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LANGUAGE: eng

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AFFILIATION: Department of Physiology and Cell Biology, University of Nevada School of Medicine, Anderson Bldg/MS352, Reno, NV 89557, USA. bperrino@medicine.nevada.edu.

Country: England

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MEDLINETA: J Physiol

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