Modulation of membrane channel currents by gap junction protein mimetic peptides: size matters.

Modulation of membrane channel currents by gap junction protein mimetic peptides: size matters. Research Abstract Details 

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Modulation of membrane channel currents by gap junction protein mimetic peptides: size matters. Abstract Text:

Junjie Wang,Meiyun Ma,Silviu Locovei,Robert W Keane,Gerhard Dahl,

Connexin mimetic peptides are widely used to assess the contribution of nonjunctional connexin channels in several processes, including ATP release. These peptides are derived from various connexin sequences and have been shown to attenuate processes downstream of the putative channel activity. Yet so far, no documentation of effects of peptides on connexin channels has been presented. We tested several connexin and pannexin mimetic peptides and observed attenuation of channel currents that is not compatible with sequence specific actions of the peptides. Connexin mimetic peptides inhibited pannexin channel currents but not the currents of the channel formed by connexins from which the sequence was derived. Pannexin mimetic peptides did inhibit pannexin channel currents but also the channels formed by connexin 46. The same pattern of effects was observed for dye transfer, except that the inhibition levels were more pronounced than for the currents. The channel inhibition by peptides shares commonalities with channel effects of polyethylene glycol (PEG), suggesting a steric block as a mechanism. PEG accessibility is in the size range expected for the pore of innexin gap junction channels, consistent with a functional relatedness of innexin and pannexin channels.

Modulation of membrane channel currents by gap junction protein mimetic peptides: size matters. Publishing Authors By Initials

J Wang,M Ma,S Locovei,RW Keane,G Dahl,

For similar animals: chordata: vertebrates: amphibia: anura: pipidae: xenopus: xenopus laevis research abstracts see: animals: chordata: vertebrates: amphibia: anura: pipidae: xenopus: xenopus laevis research

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Modulation of membrane channel currents by gap junction protein mimetic peptides: size matters. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: American journal of physiology. Cell physiology

VOLUME: 293

Page Numbers: C1112-9

Journal Abbreviation: Am. J. Physiol., Cell Physiol.

ISSN: 0363-6143

DAY: 25

MONTH: 07

YEAR: 2007

Modulation of membrane channel currents by gap junction protein mimetic peptides: size matters. Information

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LANGUAGE: eng

NlmUniqueID: 100901225

Modulation of membrane channel currents by gap junction protein mimetic peptides: size matters. Keywords Mesh Terms:

KEYWORDS: Xenopus laevis

MESH TERMS: physiology

Chemical & Substance for Abstract: Modulation of membrane channel currents by gap junction protein mimetic peptides: size matters. Information

Substance Name: Calcium

Registry Number: 7440-70-2

Grant and Affiliation Information for Modulation of membrane channel currents by gap junction protein mimetic peptides: size matters.

AFFILIATION: Dept. of Physiology and Biophysics, Univ. of Miami, School of Medicine, PO Box 016430, Miami FL 33101, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM48610

ACRONYM: GM

MEDLINETA: Am J Physiol Cell Physiol

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