Modifying the proliferative state of target cells to control DNA expression and identifying cell types transfected in vivo.

Modifying the proliferative state of target cells to control DNA expression and identifying cell types transfected in vivo. Research Abstract Details 

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Modifying the proliferative state of target cells to control DNA expression and identifying cell types transfected in vivo. Abstract Text:

Kathryn W Riddle,Hyun-Joon Kong,J Kent Leach,Claudia Fischbach,Charles Cheung,Kristi S Anseth,David J Mooney,

Although the majority of current gene transfer techniques have focused on increasing the ability of the DNA to enter the cell, it is possible that changing the proliferative and migratory state of cells will influence the cells ability to take up and express plasmid DNA. This study was designed to test the hypothesis that growth factors (basic fibroblast growth factor (bFGF) and hepatocyte growth factor/scatter factor (HGF/SF)) used to alter the proliferative and migratory state of cells can alter plasmid DNA uptake and expression. In vitro studies indicate that enhancing cell proliferation with growth factor exposure enhances plasmid DNA uptake and expression. Furthermore, dual localized delivery of bFGF and plasmid DNA in vivo increases the expression, 3-6 times over control, as compared to plasmid delivery alone. Dual delivery of a factor promoting cell proliferation and a plasmid led to a further increase in the expression of the plasmid encoding bone morphogenetic protein-2 in a rat cranial defect by specific cell populations. The results of these studies suggest that increasing the proliferative state of target cell populations can enhance non-viral gene transfer.

Modifying the proliferative state of target cells to control DNA expression and identifying cell types transfected in vivo. Publishing Authors By Initials

KW Riddle,HJ Kong,JK Leach,C Fischbach,C Cheung,KS Anseth,DJ Mooney,

For similar investigative techniques: genetic techniques: gene transfer techniques: transfection research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transfection research

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Modifying the proliferative state of target cells to control DNA expression and identifying cell types transfected in vivo. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular therapy : the journal of the American So

VOLUME: 15

Page Numbers: 361-8

Journal Abbreviation: Mol. Ther.

ISSN: 1525-0016

DAY: 3

MONTH: Feb

YEAR: 2007

Modifying the proliferative state of target cells to control DNA expression and identifying cell types transfected in vivo. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100890581

Modifying the proliferative state of target cells to control DNA expression and identifying cell types transfected in vivo. Keywords Mesh Terms:

KEYWORDS: Transfection

MESH TERMS: therapy

Chemical & Substance for Abstract: Modifying the proliferative state of target cells to control DNA expression and identifying cell types transfected in vivo. Information

Substance Name: Hepatocyte Growth Factor

Registry Number: 67256-21-7

Grant and Affiliation Information for Modifying the proliferative state of target cells to control DNA expression and identifying cell types transfected in vivo.

AFFILIATION: Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan, USA.

Country: United States

AGENCY: United States NIDCR

GRANT: R37 DE013033

ACRONYM: DE

MEDLINETA: Mol Ther

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