Modification of Rhizopus delemar lipase by its binding with phospholipids.

Modification of Rhizopus delemar lipase by its binding with phospholipids. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Modification of Rhizopus delemar lipase by its binding with phospholipids. Abstract Text:

M Iwai,Y Shimada,Y Tsujisaka,

Rhizopus delemar (ATCC 34612) lipase was modified by phospholipid (PL)-treatment so as to enhance its activity on lipoprotein. In order to detect change in lipase conformation in the modified state, a preliminary experiment was performed to remove PL from the PL-treated lipase solution which included PL nonessential to enhancement of lipoprotein lipase (LPL) activity. It was found that treatment with a mixture of isopropyl ether: n-butanol (3 : 1) was suitable for this purpose because of the stability of the enzyme. Changes in isoelectric point and alpha-helical content of lipase caused by PL-treatment were studied by means of isoelectric focusing and circular dichroism spectrum. The isoelectric point of lipase was found to shift to the acidic side on its binding with phosphatidylcholine (PC) or cardiolipin (CL). The circular dichroism spectra of the original lipase and PL-treated lipases indicated that the alpha-helical content of lipase decreased on its binding with PL. In CL-bound lipase, which was more greatly enhanced as to LPL activity than was PC-bound lipase, alpha-helical content was decreased to a larger extent than that of PC-treated lipase.

Modification of Rhizopus delemar lipase by its binding with phospholipids. Publishing Authors By Initials

M Iwai,Y Shimada,Y Tsujisaka,

For similar fungi: zygomycota: mucorales: rhizopus research abstracts see: fungi: zygomycota: mucorales: rhizopus research

PUBMED ID PMID:

MEDLINE DATE:

Modification of Rhizopus delemar lipase by its binding with phospholipids. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 88

Page Numbers: 533-8

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1980

Modification of Rhizopus delemar lipase by its binding with phospholipids. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Modification of Rhizopus delemar lipase by its binding with phospholipids. Keywords Mesh Terms:

KEYWORDS: Rhizopus

MESH TERMS: enzymology

Chemical & Substance for Abstract: Modification of Rhizopus delemar lipase by its binding with phospholipids. Information

Substance Name: Lipoprotein Lipase

Registry Number: EC 3.1.1.34

Grant and Affiliation Information for Modification of Rhizopus delemar lipase by its binding with phospholipids.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Modification of Rhizopus delemar lipase by its binding with phospholipids Related Publications

• Increase in the activity of Rhizopus delemar lipase on water-soluble esters by its binding with phosphatidylcholine.
• Low resolution crystal structure of lipase from Geotrichum candidum (ATCC34614).
• Modification of Rhizopus delemar lipase by its binding with phospholipids.
• Modification of carboxyl groups in Geotrichum candidum lipase.
• Purification and properties of partial glyceride hydrolase of Penicillium cyclopium M1.
• Reversibility of the modification of Rhizopus delemar lipases by phosphatidylcholine.
• [Nursing report. Observation on the family relationship as part of nursing actions]