Modification of carboxyl groups in Geotrichum candidum lipase.

Modification of carboxyl groups in Geotrichum candidum lipase. Research Abstract Details 

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Modification of carboxyl groups in Geotrichum candidum lipase. Abstract Text:

A Sugihara,M Iwai,Y Tsujisaka,

Lipase from Geotrichum (Geo.) candidum was rapidly inactivated by incubation with water-soluble carbodiimide, 1-ethyl-3-dimethylaminopropyl carbodiimide (EDC), or 1-cyclohexyl-3-(2-morpholinyl-(4)-ethyl) carbodiimide metho-p-toluenesulfonate (CMC), at pH 4.8. The pH dependence of the rate of inactivation was consistent with the modification of carboxyl groups in the lipase. Reaction of the lipase with EDC in the presence of the nucleophile taurine showed that about 9 carboxyl groups per molecule of enzyme were modified with concomitant total loss of activity. This number was reduced to 4 when CMC was used as a carbodiimide instead of EDC. The modification had no effect on the CD spectrum in the ultraviolet region. Kinetic analysis of the effect of CMC on the lipase indicated that at least 1 CMC molecule bound to the enzyme during inactivation.

Modification of carboxyl groups in Geotrichum candidum lipase. Publishing Authors By Initials

A Sugihara,M Iwai,Y Tsujisaka,

For similar fungi: mitosporic fungi research abstracts see: fungi: mitosporic fungi research

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Modification of carboxyl groups in Geotrichum candidum lipase. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 91

Page Numbers: 507-13

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1982

Modification of carboxyl groups in Geotrichum candidum lipase. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Modification of carboxyl groups in Geotrichum candidum lipase. Keywords Mesh Terms:

KEYWORDS: Mitosporic Fungi

MESH TERMS: enzymology

Chemical & Substance for Abstract: Modification of carboxyl groups in Geotrichum candidum lipase. Information

Substance Name: Lipase

Registry Number: EC 3.1.1.3

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Country: JAPAN

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MEDLINETA: J Biochem

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