Model proficiency testing scheme for serological diagnosis of Brucellosis: interlaboratory study.

Model proficiency testing scheme for serological diagnosis of Brucellosis: interlaboratory study. Research Abstract Details 

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Model proficiency testing scheme for serological diagnosis of Brucellosis: interlaboratory study. Abstract Text:

A model interlaboratory testing scheme was developed by the Italian National Reference Laboratory for Brucellosis. This scheme was planned for both qualitative (Rose Bengal Plate Test; RBPT) and quantitative (Complement Fixation Test; CFT) serological tests and involved a total of 42 laboratories. In the preparation of this scheme, reference was made to general protocols and guidelines and to methods reported in the literature, which were applicable to analytical chemistry laboratories. Six field sera from naturally infected animals, one positive serum at a titer below the European Union (EU) positivity threshold, and 5 sera positive at titers between 20 and 851 International Units of Complement Fixation Test (IUCFT)/mL plus one negative serum were used to produce a panel of test sera. To evaluate laboratory performances in the quantitative test for each tested sample examined, z-scores based on robust summary statistics (the median and normalized interquartile range) were used. To evaluate overall laboratory performance, 2 types of combined z-scores were used: Rescaled Sum of Scores and Sum of Squared Scores. In the case of the qualitative test (RBPT), results were analyzed by a Bayesian approach. A Beta distribution, based on the result of each laboratory, was calculated and used to estimate the probability of each laboratory giving a correct result and its uncertainty.

Model proficiency testing scheme for serological diagnosis of Brucellosis: interlaboratory study. Publishing Authors By Initials

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Model proficiency testing scheme for serological diagnosis of Brucellosis: interlaboratory study. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of AOAC International

VOLUME: 87

Page Numbers: 965-71

Journal Abbreviation: J AOAC Int

ISSN: 1060-3271

DAY: 17

MONTH: 03

YEAR: 2008

Model proficiency testing scheme for serological diagnosis of Brucellosis: interlaboratory study. Information

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LANGUAGE: eng

NlmUniqueID: 9215446

Model proficiency testing scheme for serological diagnosis of Brucellosis: interlaboratory study. Keywords Mesh Terms:

KEYWORDS: Serology

MESH TERMS: virology

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Grant and Affiliation Information for Model proficiency testing scheme for serological diagnosis of Brucellosis: interlaboratory study.

AFFILIATION: Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise Via Campo Boario, Teramo, Italy.

Country: United States

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MEDLINETA: J AOAC Int

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