Mode of inhibition of ornithine aminotransferase by L-canaline.

Mode of inhibition of ornithine aminotransferase by L-canaline. Research Abstract Details 

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Mode of inhibition of ornithine aminotransferase by L-canaline. Abstract Text:

K Kito,Y Sanada,N Katunuma,

The mechanism of inhibition of ornithine aminotransferase [EC 2.6.1.13] by L-canaline (alpha-amino-gamma-amino-oxybutyric acid) was investigated. Spectral changes of pyridoxal 5'-phosphate in ornithine aminotransferase on addition of L-canaline showed that L-canaline formed an oxime-type compound with pyridoxal 5'-phosphate that had the same spectra as the compound formed on addition of hydroxylamine to the holoenzyme. Kinetic studies indicated that hydroxylamine was a reversible noncompetitive inhibitor, whereas L-canaline was an irreversible inhibitor of ornithine aminotransferase. Other analogs, such as delta-aminovaleric acid and alpha-N-acetyl-L-ornithine, also reacted with the pyridoxal 5'-phosphate of the enzyme, but these compounds were competitive inhibitors with respect to L-ornithine. L-Canaline and hydroxylamine also reacted with pyridoxal 5'-phosphate in pig heart aspartate aminotransferase [EC 2.6.1.1] to produce an oxime, but both of them were reversible and noncompetitive inhibitors of the enzyme. The Ki value of hydroxylamine for ornithine aminotransferase was 4.3 X 10(-7) M and those of L-canaline and hydroxylamine for aspartate aminotransferase were 1.7 X 10(-4) M and 2.2 X 10(-5) M, respectively.

Mode of inhibition of ornithine aminotransferase by L-canaline. Publishing Authors By Initials

K Kito,Y Sanada,N Katunuma,

For similar organic chemicals: carboxylic acids: acids, acyclic: valerates research abstracts see: organic chemicals: carboxylic acids: acids, acyclic: valerates research

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Mode of inhibition of ornithine aminotransferase by L-canaline. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 83

Page Numbers: 201-6

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1978

Mode of inhibition of ornithine aminotransferase by L-canaline. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Mode of inhibition of ornithine aminotransferase by L-canaline. Keywords Mesh Terms:

KEYWORDS: Valerates

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Mode of inhibition of ornithine aminotransferase by L-canaline. Information

Substance Name: Ornithine-Oxo-Acid Transaminase

Registry Number: EC 2.6.1.13

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Country: JAPAN

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MEDLINETA: J Biochem

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